低氧暴露下高原鼠兔肺组织间隙连接蛋白40表达分析

doi:10.16829/j.slxb.150686

兽类学报 ›› 2022, Vol. 42 ›› Issue (5): 572-578.DOI: 10.16829/j.slxb.150686

低氧暴露下高原鼠兔肺组织间隙连接蛋白40表达分析

张湑泽¹(), 付林²^,⁵, 邹小艳³, 都玉蓉⁴()

^1.青海民族大学生态环境与资源学院，国家民委青藏高原资源化学与生态环境保护重点实验室，西宁 810007
^2.云南大学生命科学学院，昆明 650091
^3.长沙医学院药学院，长沙 410219
^4.青海师范大学生命科学学院，青海省青藏高原生物多样性形成机制与综合利用重点实验室，西宁 810008
^5.中国科学院西北高原生物研究所，中国科学院高原生物适应与进化重点实验室，西宁 810001

收稿日期:2022-04-23 接受日期:2022-08-19 出版日期:2022-09-30 发布日期:2022-09-21
通讯作者: 张湑泽,都玉蓉
作者简介:付林 (1991- )，男，博士研究生，主要从事为动物功能代谢研究.第一联系人：共同第一作者
基金资助:
中国科学院青海省人民政府三江源国家公园联合研究专项(LHZX-2020-01);青海省自然科学基金(2020-ZJ-965Q);青海民族大学校级规划项目(2021XJGH15)

Expression analysis of gap junction protein 40 in lung of plateau pika exposed to hypoxia

Xuze ZHANG¹(), Lin FU²^,⁵, Xiaoyan ZOU³, Yurong DU⁴()

^1.College of Eco-Environment and Resources, Key Laboratory of Resource Chemistry and Eco-environmental Protection in Tibetan Plateau of State Ethnic Affairs Commissionm, Qinghai Minzu University, Xining 810007, China
^2.College of Life Sciences, Yunnan University, Kunming 650091, China
^3.College of Pharmaceutical, Changsha Medical University, Changsha 410219, China
^4.College of Life Sciences Qinghai Normal University, Key Laboratory of Biodiversity Formation Mechanism and Comprehensive Utilization of Qinghai-Tibetan Plateau in Qinghai Province, Xining 810008, China
^5.Key Laboratory of Adaptation and Evolutionof Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China

Received:2022-04-23 Accepted:2022-08-19 Online:2022-09-30 Published:2022-09-21
Contact: Xuze ZHANG,Yurong DU

摘要/Abstract

摘要：

低氧性肺血管收缩反应钝化是高原鼠兔适应低氧环境的重要策略，但参与该生理代偿反应的功能基因尚不明确。间隙连接蛋白40 (Connexin40, Cx40) 在哺乳动物肺血管内皮表达。本研究对生活在海拔3 200 m的高原鼠兔进行28 d模拟海拔5 000 m低氧处理，以Sprague Dawley (SD) 大鼠为对照，采用免疫组化法分析模拟低氧处理后高原鼠兔和SD大鼠肺组织形态结构，qPCR和蛋白印记法检测Cx40基因和蛋白表达量变化，探究Cx40在高原鼠兔低氧性肺血管收缩反应钝化中的潜在作用。结果显示，低氧处理后，高原鼠兔肺泡呈空泡囊状，Cx40蛋白在支气管和肺血管中均表达，Cx40 基因mRNA水平随着低氧暴露而升高，但其蛋白质水平呈下降趋势，肺支气管Cx40蛋白无明显变化。SD大鼠肺血管和肺支气管表达的Cx40蛋白均无明显变化。暗示生活在高海拔低氧环境中的高原鼠兔，Cx40蛋白下调可抑制血管收缩信号，减弱低氧性肺血管收缩反应，使低氧性肺血管收缩反应钝化，以适应高原缺氧环境。研究结果可为高原土著动物适应高寒缺氧环境提供基础理论数据。

关键词: 青藏高原, 低氧, 低氧性肺血管收缩反应钝化, 高原鼠兔, 间隙连接蛋白40

Abstract:

Blunted hypoxic pulmonary vasoconstriction response is a major adaptation to hypoxia in plateau pika (Ochotona curzoniae). However, the genes involved in this physiological compensation remained unclear. In the present study, plateau pikas and Sprague Dawley (SD) rats were challenged with a hypoxia condition simulating an altitude of 5 000 m for 28 days. Morphological structure analysis by immunohistochemistry was carried out for the pulmonary in both species and the expression levels of Connexin40 (Cx40), a member of the Connexon family expressed in pulmonary vascular endothelia in mammals, were determined by both qPCR and Western blot. The results showed that upon hypoxia challenge the alveoli of plateau pika appeared to be vacuolated sacs. Cx40 expression was detected in both bronchi and pulmonary blood vessels in both species. In plateau pikas, mRNA level of Cx40 increased after hypoxia challenge, while its protein level decreased. In contrast, protein levels of Cx40 had no significant change with prolonged hypoxia exposure in pulmonary blood vessels of SD rats. No significant change in the Cx40 protein in the lung bronchi was observed in both species. Our results indicate that with hypoxia exposure, pulmonary vasoconstriction response in plateau pikas could be suppressed by down-regulation of Cx40, contributing to their adaptation to a hypoxic environment on the plateau. Our results can provide a reference for the expression analysis of gap junction proteins in the lungs of indigenous animals living on the Qinghai-Tibet Plateau.

Key words: Qinghai-Tibet Plateau, Hypoxia, Blunted hypoxia pulmonary vasoconstriction, Plateau pika (Ochotona curzoniae), Connexin40

中图分类号:

张湑泽, 付林, 邹小艳, 都玉蓉. 低氧暴露下高原鼠兔肺组织间隙连接蛋白40表达分析[J]. 兽类学报, 2022, 42(5): 572-578.

Xuze ZHANG, Lin FU, Xiaoyan ZOU, Yurong DU. Expression analysis of gap junction protein 40 in lung of plateau pika exposed to hypoxia[J]. ACTA THERIOLOGICA SINICA, 2022, 42(5): 572-578.

图/表 3

图1 低氧处理前后高原鼠兔和SD大鼠肺组织Cx40蛋白的免疫组化定位. A ~ B：SD大鼠肺组织切片；C ~ D：高原鼠兔肺组织切片；E ~ F：SD大鼠低氧处理后肺组织切片；G ~ H：高原鼠兔低氧处理后肺组织切片. 图中红色箭头指向血管，蓝色箭头指向支气管

Fig. 1 Cx 40 localization in the lung tissues of plateau pika and SD Rat between hypoxia group and control group. A - B: refers lung tissue sections of SD rats; C - D: refers lung tissue sections of plateau pika; E - F: refers lung tissue sections of SD rats treated with hypoxia; G - H: refers lung tissue sections of plateau pika treated with hypoxia. The red arrow point to vascular, the blue arrow points to the bronchus. The red arrow point to vascular, the blue arrow points to the bronchus

图2 高原鼠兔低氧处理前后Cx40基因mRNA表达量 (n = 6). mRNA定量以GAPDH为内参基因，结果以mean ± SE表示，*表示显著差异 (P < 0.05)

Fig. 2 Cx40 mRNA expression level in plateau pikas treated with hypoxia (n = 6). GAPDH was used as the reference gene for mRNA quantification, and the result was expressed by mean ± SE. * represents significant difference (P < 0.05)

图3 高原鼠兔和SD大鼠在低氧处理前后Cx40蛋白水平的变化(n = 3). 蛋白定量以β-actin为内参，结果以mean ± SE表示. **表示极显著差异 (P < 0.01)，ns表示无显著差异

Fig. 3 Changes of Cx40 protein levels in plateau pika and SD rat under hypoxia treatment (n = 3). β-actin was used as internal reference for protein quantification, and the results were expressed as mean ± SE; ** represented extremely significant difference (P < 0.01), while ns represented no significant difference

参考文献 0

	Bolanos J P, Medina J M. 1996. Induction of nitric oxide synthase inhibits gap junction permeability in cultured rat astrocytes. Journal of Neurochemistry, 66 (5): 2091-2099.
	Bruzzone R, Haefliger J, Gimlich R, Paul D L. 1993. Connexin40, a component of gap junctions in vascular endothelium, is restricted in its ability to interact with other connexins. Molecular Biology of the Cell, 4 (1): 7-20.
	Chen Q H. 2001. The changes of function and morphology of pulmonary arterial vessels in the pika at high altitude. China Journal Applied Physiology, 17 (2): 178-181. (in Chinese)
	Chen Q S, Feng X, Jiang S C. 2006. Structural study on plateau adaptability of yak lung. Scientia Agricultura Sinica, 39 (10): 2107-2113. (in Chinese)
	Cowburn A S, Crosby A, Macias D, Branco C, Colaço R, Southwood M, Toshner M, Crotty Alexander L E, Morrell N W, Chilvers E R, Johnson R S. 2016. HIF2α-arginase axis is essential for the development of pulmonary hypertension. Proceedings of the National Academy of Sciences of the United States of America, 113 (31): 8801.
	Du J Z, Li Q F. 1982. Effects of simulated hypoxic acclimation on organism, orgin and hematology in Ochotona curzoniae and rats. Acta Theriologica Sinica, 2 (1): 35-42. (in Chinese)
	Feng Z J, Zheng C L. 1985. Studies on the pikas (genus: Ochotona) of China‒Taxonomic notes and distribution. Acta Theriologica Sinica, 5 (4): 269-289. (in Chinese)
	Fu L. 2018. Comparative transcriptome analysis of plateau pika (Ochotona curzoniae) bHPV related genes and initial verification.Master thesis. Beijing: University of Chinese Academy of Sciences. (in Chinese)
	Gabriels J E, Paul D L. 1998. Connexin43 is highly localized to sites of disturbed flow in rat aortic endothelium but connexin37 and connexin40 are more uniformly distributed. Circulation Research, 83 (6): 636-643.
	Ge R L, Kubo K, Kobayashi T, Sekiguchi M, Honda T. 1998. Blunted hypoxic pulmonary vasoconstrictive response in the rodent Ochotona curzoniae (pika) at high altitude. The American Journal of Physiology, 274: 1792-1799.
	Grilli A, De Lutiis M A, Patruno A, Speranza L, Cataldi A, Centurione L, Taccardi A A, Di Napoli P, De Caterina R, Barbacane R, Conti P, Felaco M. 2003. Effect of chronic hypoxia on inducible nitric oxide synthase expression in rat myocardial tissue. Experimental Biology and Medicine, 228 (8): 935-942.
	Hervé J C, Derangeon M. 2013. Gap-junction-mediated cell-to-cell communication. Cell & Tissue Research, 352 (1): 21-31.
	Kumar N M, Gilula N B. 1996. The gap junction communication channel. Cell, 84 (3): 381-388.
	Leybaert L, Lampe P D, Dhein S, Kwak B R, Ferdinandy P, Beyer E C, Laird D W, Naus C C, Green C R, Schulz R. 2017. Connexins in cardiovascular and neurovascular health and disease: Pharmacological implications. Pharmacological Reviews, 69 (4): 396-478.
	Li H G, Ren Y M, Guo S C, Cheng L, Wang D P, Yang J, Chang Z J, Zhao X Q. 2009. The protein level of hypoxia‐inducible factor‐1α is increased in the plateau pika (Ochotona curzoniae) inhabiting high altitudes. Journal of Experimental Zoology Part A Ecological Genetics & Physiology, 311 (2): 134-141.
	Li S, Zou X Y, Fu L, Liu Z H, Bai X H, Du Y R, Guo S C. 2020. Comparative on pulmonary histochemical characteristics between plateau pika and Kunming mouse. Acta Theriologica Sinica, 40 (2): 162-169. (in Chinese)
	Lu C, Mcmahon D G. 1997. Modulation of hybrid bass retinal gap junctional channel gating by nitric oxide. The Journal of Physiology, 499: 689-699.
	Ma L, Ge R L. 2007. Molecular mechanism of adaptation to hypoxia in pika. Progress in Physiological Sciences, 38 (2): 143-146. (in Chinese)
	Pan L J, Li X Z, Zhuo H Y, Li L, Wei L L, Yu X S, Si J Q, Ma K T. 2015. Difference expression of Cx37 and Cx40 of arteries in spontaneously hypertensive rats. Chongqing Medicine, 44 (1): 4-6. (in Chinese)
	Rudyk O, Rowan A, Prysyazhna O, Krasemann S, Hartmann K, Zhang M, Shah A M, Ruppert C, Weiss A, Schermuly R T, Ida T, Akaike T, Zhao L, Eaton P. 2019. Oxidation of PKGIα mediates an endogenous adaptation to pulmonary hypertension. Proceedings of the National Academy of Sciences of the United States of America, 116 (26): 13016-13025.
	Semenza G L. 2005. New insights into nNOS regulation of vascular homeostasis. The Journal of Clinical Investigation, 115: 2976-2978.
	Severs N J, Rothery S, Dupont E, Coppen S R, Yeh H I, Ko Y S, Matsushita T, Kaba R, Halliday D. 2001. Immunocytochemical analysis of connexin expression in the healthy and diseased cardiovascular system. Microscopy Research and Technique, 52 (3): 301-322.
	Sheafor B A. 2003. Metabolic enzyme activities across an altitudinal gradient: an examination of pikas (genus Ochotona). The Journal of Experimental Biologyl, 206: 1241-1249.
	Si R, Zhang Q, Cabrera J, Zheng Q, Tsuji‑Hosokawa A, Watanabe M, Hosokawa S, Xiong M, Jain P P, Ashton A W, Yuan J X, Wang J, Makino A. 2020. Chronic hypoxia decreases endothelial connexin40, attenuates endothelium-dependent hyperpolarization-mediated relaxation in small distal pulmonary arteries, and leads to pulmonary hypertension. Journal of the American Heart Association, 9 (24): e018327.
	Simon A M, McWhorter A R, Chen H, Jackson C L, Ouellette Y. 2004. Decreased intercellular communication and connexin expression in mouse aortic endothelium during lipopolysaccharide-induced inflammation. Journal of Vascular Research, 41 (4): 323-333.
	Totland M Z, Rasmussen N L, Knudsen L M, Leithe E. 2020. Regulation of gap junction intercellular communication by connexin ubiquitination: physiological and pathophysiological implications. Cellular and Molecular Life Sciences, 77 (4): 573-591.
	Voelkel N F, Mizuno S, Bogaard H J. 2013. The role of hypoxia in pulmonary vascular diseases: a perspective. The Journal of Clinical Investigation, 304: 457-465.
	Wang L, Yin J, Nickles H T, Ranke H, Tabuchi A, Hoffmann J, Tabeling C, Barbosa‑Sicard E, Chanson M, Kwak B R, Shin H S, Wu S, Isakson B E, Witzenrath M, de Wit C, Fleming I, Kuppe H, Kuebler W M. 2012. Hypoxic pulmonary vasoconstriction requires connexin 40‒mediated endothelial signal conduction. The Journal of Clinical Investigation, 122 (11): 4218-4230.
	Wang R, Pan J J, Wang D Y, Ji Y K, Li M C, Jin H F, Yuan Y H. 2021. ‘Blue lips’: research progress in pulmonary vascular remodeling in hypoxic pulmonary hypertension. Scientia Sinica Vitae, 51: 1637-1645. (in Chinese)
	Wang T, Zhang Z X, Liu S Y, Wu T Y, Sun B Y, Wang D X. 2004. Role of prostaglandins nitric oxide and potassium channel in the blunting of hypoxic pulmonary vasoconstriction in the high altitude adaptive animal. Chinese Journal of Pathophysiology, 20 (5): 711-714. (in Chinese)
	Wang X J, Wei D B, Wei L, Zhang J M, Yu H Y. 2008. Physiological character of erythrocyte adapting to hypoxia in plateau zokor and platean pika. Sichuan Journal of Zoology, 27 (6): 1100-1103. (in Chinese)
	Wang X Q, Wang Z G, Chen Q H, Wang Z G, Wen J L. 2001. Changes on pulmonary arterial pressure and level of NO in pika. Journal of High Altitude Medicine, 11 (1): 2-6. (in Chinese)
	Weigert A. 1996. Endothelium-dependent vascular hyporesponsiveness without nitric oxide synthase induction in aorta of cirrhotic rats. Hepatology, 22 (6): 1856-1862.
	Wit C D, Roos F, Bolz S S, Kirchhoff S, Krüger O, Willecke K, Pohl U. 2000. Impaired conduction of vasodilation along arterioles in connexin40-deficient mice. Circulation Research, 86 (6): 649-655.
	Wölfle S E, Schmidt V J, Hoepfl B, Gebert A, Alcoléa S, Gros D, Wit C D. 2007. Connexin45 cannot replace the function of connexin40 in conducting endothelium-dependent dilations along arterioles. Circulation Research, 101 (12): 1292.
	Wu Y, Du J Z. 2001. Study of activity of HPA in rat and Ochotona curzoniae under hypoxia. Acta Theriologica Sinica, 21 (3): 195-198. (in Chinese)
	Xie L, Zhang X Z, Qi D L, Guo X Y, Pang B, Dou Y R, Zou X Y, Guo S C, Zhao X Q. 2014. Inhibition of inducible nitric oxide synthase expression and nitric oxide production in plateau pika (Ochotona curzoniae) at high altitude on Qinghai-Tibet Plateau. Nitric Oxide: Biology and Chemistry, 38: 38-44.
	Zhang J J, Chen J H, Zhao M P, Wu H L, Zhang C C, Ying L, Chen X W, Wang W Y. 2018. The role of endoplasmic reticulum stress in pulmonary hypertension in rat induced by chronic hypoxia and hypercapnia. Chinese Journal of Applied Physiology, 34 (4): 327-333, 387. (in Chinese)
	Zhang X Z, Fu L, Zou X Y, Li S, Ma X D, Xie L, Pang B, Ma J B, Wang Y J, Dou Y R, Guo S C. 2022. Lung transcriptome analysis for the identification of genes involved in the hypoxic adaptation of plateau pika (Ochotona curzoniae). Comparative Biochemistry and Physiology‒Part D: Genomics and Proteomics, 41: 100943.
	Zhao T B, Ning H X, Zhu S S, Sun P, Xu S X, Chang Z J, Zhao X Q. 2004. Cloning of hypoxia-inducible factor 1α cDNA from a high hypoxia tolerant mammal-plateau pika (Ochotona curzoniae). Biochemical and Biophysical Research Communications, 316: 565-572.
	Zhuo H Y, Pan L J, Li X Z, Li L, Wei L L, Yu X S, Si J Q, Ma K T. 2014. Difference expression of Cx43 and Cx45 in aorta of spontaneously hypertensive rats and normotensive rats. China Journal of Modern Medicine, 24 (25): 1-4. (in Chinese)
	马兰, 格日力. 2007. 高原鼠兔低氧适应分子机制的研究进展. 生理科学进展, 38 (2): 143-146.
	王晓君, 魏登邦, 魏莲, 张建梅, 于红妍. 2008. 高原鼢鼠和高原鼠兔红细胞低氧适应特征. 四川动物, 27 (6): 1100-1103.
	王晓勤, 王占刚, 陈秋红, 王志刚, 温佳林. 2001. 高原鼠兔肺动脉压与NO的变化. 高原医学杂志, 11 (1): 2-6.
	王睿, 潘进进, 王定友, 姬雨轲, 刘明成, 金海峰, 袁予辉. 2021. “蓝嘴唇”: 缺氧性肺动脉高压肺血管重构研究进展. 中国科学: 生命科学, 51 (12): 1637-1645.
	付林. 2018. 高原鼠兔bHPV相关基因的比较转录组分析与初步验证. 北京: 中国科学院大学硕士学位论文.
	冯祚建, 郑昌琳. 1985. 中国鼠兔属 (Ochotona) 的研究: 分类与分布. 兽类学报, 5 (4): 269-289.
	杜继曾, 李庆芬. 1982. 模拟高原低氧对高原鼠兔和大鼠器官与血液若干指标的影响. 兽类学报, 2 (1): 35-42.
	李双, 邹小艳, 付林, 刘忠浩, 白祥慧, 都玉蓉, 郭松长. 2020. 高原鼠兔和昆明白小鼠肺组织结构比较. 兽类学报, 40 (2): 162-169.
	陈秋红. 2001. 高原鼠兔肺动脉血管功能及形态变化.中国应用生理学杂志, 17 (2): 178-181.
	陈秋生, 冯霞, 姜生成. 2006. 牦牛肺脏高原适应性的结构研究.中国农业科学, 39 (10): 2107-2113.
	吴雁, 杜继曾. 2001. 低氧暴露条件下高原鼠兔和大鼠HPA轴活动的比较. 兽类学报, 21 (3): 195-198.
	汪涛, 张珍祥, 刘声远, 吴天一, 孙秉庸, 王迪浔. 2004. 前列腺素、一氧化氮和钾通道在鼠兔缺氧肺血管反应钝化中的作用. 中国病理生理杂志, 20 (5): 711-714.
	张晶晶, Chen J H, 赵美平, 武垣伶, 张聪聪, 应磊, 陈锡文, 王万铁. 2018. 内质网应激在大鼠低氧高二氧化碳性肺动脉高压中的作用. 中国应用生理学杂志, 34 (4): 327-333, 387.
	卓泓宇, 潘立君, 李新芝, 李丽, 魏丽丽, 于秀石, 司军强, 马克涛. 2014. Cx43和Cx45在自发性高血压和正常血压大鼠主动脉的表达差异. 中国现代医学杂志, 24 (25): 1-4.
	潘立君, 李新芝, 卓泓宇, 李丽, 魏丽丽, 于秀石, 司军强, 马克涛. 2015. Cx37和Cx40在自发性高血压大鼠不同动脉间的表达差异. 重庆医学, 44 (1): 4-6.

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低氧暴露下高原鼠兔肺组织间隙连接蛋白40表达分析

Expression analysis of gap junction protein 40 in lung of plateau pika exposed to hypoxia

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