关键词: 川金丝猴, 类固醇激素, 一致性, 酶联免疫吸附测定法

Abstract: Steroid hormone metabolites in the feces and urine (SHMFU)have been used to monitor the physiological status,i. e. ,reproductive,health or social status,in many primate species. Owing to the different metabolic path in the body,SHMFU may have different concentration peak. The refore,it is necessary to analyze the correlation or consistency of SHMFU when apply them to identify the physiological status of the animal. In this study,we clarify the consistency of SHMFU of a semi-free ranging group in golden snub-nosed monkeys (Rhinopithecus roxellana) at Shennongjia,central China,with total 84 samples,including two adult males (24 samples)and ten adult females (60 samples),from November,
2011 to June,2012. Enzyme-linked immunoassays (EIA)were applied to detect the testosterone of the male,testosterone estradiol and progesterone of the female. Significant correlation of testosterone,testosterone estradiol and progesterone in the feces and urine were found both the males and females. The result indicated that it was feasible to judge the physiological status of R. roxellana through SHMFU. However,this study couldn’t provide the lag time of SHMFU because the number of samples is not enough.

Key words: Correlation, EIA, Golden snub-nosed monkey (Rhinopithecus roxellana), Steroid hormone