兽类学报 ›› 2026, Vol. 46 ›› Issue (2): 153-170.DOI: 10.16829/j.slxb.151026
• 研究论文 • 下一篇
布威海丽且姆·阿巴拜科日1,2,3,4(
), 艾比布拉·阿布都拉1,2, 布海力切木·依明艾力1,2, 赵华斌4(
)
收稿日期:2024-11-06
接受日期:2025-04-18
出版日期:2026-03-30
发布日期:2026-03-06
通讯作者:
布威海丽且姆·阿巴拜科日,赵华斌
基金资助:
Buweihailiqiemu ABABAIKERI1,2,3,4(
), Aibibula ABUDULA1,2, Buhailiqiemu YIMINGAILI1,2, Huabin ZHAO4(
)
Received:2024-11-06
Accepted:2025-04-18
Online:2026-03-30
Published:2026-03-06
Contact:
Buweihailiqiemu ABABAIKERI, Huabin ZHAO
摘要:
为研究塔里木马鹿(Cervus elaphus yarkandensis)干旱环境适应中可能具有抗氧化功能的过氧化物还原酶1(Peroxiredoxins 1,PRDX1)的结构与功能特征,本研究通过逆转录PCR技术(RT‑PCR)扩增获取塔里木马鹿PRDX1(CeyPRDX1)基因的编码序列(CDS),使用同源重组法将其与pcDNA3.1(+) 载体相连接,并构建其真核表达载体,命名为pcDNA3.1(+)‑CeyPRDX1;利用具有EGFP荧光标签的pLJM1‑EGFP慢病毒骨架载体构建慢病毒过表达载体(pLJM1‑CeyPRDX1‑EGFP),并感染人永生化角质形成细胞株HaCaT后检测其亚细胞定位。对CeyPRDX1与其他哺乳动物的PRDX1基因序列进行对位排列,构建系统进化树;利用生物信息学方法对CeyPRDX1蛋白理化性质、跨膜结构、修饰位点和亚细胞定位等进行分析。结果显示,CeyPRDX1基因的CDS序列为729 bp,成功构建其真核表达载体和慢病毒过表达载体;亚细胞定位显示,CeyPRDX1定位于细胞质和细胞核中。对外排列和系统进化树分析结果显示,CeyPRDX1与东欧马鹿(Cervus elaphus)以及马鹿(Cervus canadensis)的同源性最高,亲缘关系最近。生物信息学分析结果显示,CeyPRDX1是高度保守的不稳定蛋白,没有N‑糖基化位点和跨膜区,其蛋白与多种抗氧化相关蛋白之间存在相互作用。本研究结果为后续CeyPRDX1基因的功能研究奠定了基础。
中图分类号:
布威海丽且姆·阿巴拜科日, 艾比布拉·阿布都拉, 布海力切木·依明艾力, 赵华斌. 塔里木马鹿PRDX1基因表达载体的构建及其生物信息学分析[J]. 兽类学报, 2026, 46(2): 153-170.
Buweihailiqiemu ABABAIKERI, Aibibula ABUDULA, Buhailiqiemu YIMINGAILI, Huabin ZHAO. Construction of the expression vector for the PRDX1 gene in Tarim red deer and its bioinformatic analysis[J]. ACTA THERIOLOGICA SINICA, 2026, 46(2): 153-170.
| 基因名称 Gene name | 引物序列(5′→3′) Primer sequence | 用途 Utilization |
|---|---|---|
塔里木马鹿PRDX1 PRDX1 gene in Tarim red deer | F(BamHⅠ): ttaagcttggtaccgagctc GGGTAAG; R(EcoRⅠ): actgtgctggatatctgca | 真核表达载体构建 Construction of eukaryoticexpression vectors |
塔里木马鹿PRDX1 PRDX1 gene in Tarim red deer | F: CCCTCTTGACTTCACCTT; R: GTCCTCCTTGTTTCTTGG | qRT-PCR检测 qRT-PCR detection |
| GAPDH | F: TCAAGAAGGTGGTGAAGCAGG; R: GCGTCAAAGGTGGAGGAGTG |
表1 引物序列信息
Table 1 Primer sequence information
| 基因名称 Gene name | 引物序列(5′→3′) Primer sequence | 用途 Utilization |
|---|---|---|
塔里木马鹿PRDX1 PRDX1 gene in Tarim red deer | F(BamHⅠ): ttaagcttggtaccgagctc GGGTAAG; R(EcoRⅠ): actgtgctggatatctgca | 真核表达载体构建 Construction of eukaryoticexpression vectors |
塔里木马鹿PRDX1 PRDX1 gene in Tarim red deer | F: CCCTCTTGACTTCACCTT; R: GTCCTCCTTGTTTCTTGG | qRT-PCR检测 qRT-PCR detection |
| GAPDH | F: TCAAGAAGGTGGTGAAGCAGG; R: GCGTCAAAGGTGGAGGAGTG |
图 1 塔里木马鹿PRDX1基因的RT-PCR扩增和重组质粒的构建. A:RT-PCR扩增电泳图. M为marker;1、2和3为塔里木马鹿皮肤组织;4为阴性对照;B:pcDNA3.1(+)-CeyPRDX1重组载体的质粒图谱
Fig. 1 The RT-PCR amplification of PRDX1 gene in Tarim red deer and construction of recombinant plasmid. A: The electropherogram of RT-PCR amplification. M is marker; Lane 1, 2 and 3 are skin tissues of Tarim red deer; Lane 4 is the negative control; B: Plasmid mapping of pcDNA3.1(+)-CeyPRDX1 recombinant vector
图 3 pcDNA3.1(+)-CeyPRDX1重组载体酶切鉴定结果. M为1 kb marker;1为重组载体酶切产物;2为重组载体;3为阴性对照
Fig. 3 Restriction enzyme digestion result of pcDNA3.1(+)-CeyPRDX1. M is the 1 kb marker; 1 is the enzyme digestion product of recombinant vector; 2 is the pcDNA3.1(+)-CeyPRDX1 recombinant vector; 3 is the negative control
图 4 pLJM1-CeyPRDX1-EGFP重组载体的质粒图谱及其酶切鉴定结果. A:pLJM1-CeyPRDX1-EGFP重组载体的质粒图谱;B:重组载体酶切鉴定电泳图. M为1 kb marker;1为重组载体;2为重组载体Ecor1单酶切产物
Fig. 4 The plasmid map and restriction enzyme digestion identification result of pLJM1-CeyPRDX1-EGFP recombinant vector. A: The plasmid map of the pLJM1-CeyPRDX1-EGFP recombinant vector; B: Restriction enzyme digestion identification electrophoresis image of recombinant vector. M is the 1 kb marker; 1 is the recombinant vector; 2 is the Ecor1 single enzyme digestion product of recombinant vector
图 5 在慢病毒感染和未感染的HaCaT细胞中EGFP绿色荧光的检测. A:HaCaT细胞对照组(NC,空白组)(× 400);B:感染pLJM1-EGFP 的HaCaT细胞组(pLJM1-EGFP-vector,空载组)(× 400);C:感染pLJM1-CeyPRDX1-EGFP 的HaCaT细胞组(pLJM1-CeyPRDX1-EGFP,CeyPRDX1过表达组)(× 400)
Fig. 5 Detection of EGFP green fluorescence in lentivirus-infected and uninfected HaCaT cells. A: HaCaT cell control group (NC, blank group)(× 400); B: HaCaT cell group infected with pLJM1-EGFP(pLJM1-EGFP-vector, empty group) (× 400); C: HaCaT cell group infected with pLJM1-CeyPRDX1-EGFP (pLJM1-CeyPRDX1-EGFP, CeyPRDX1 overexpression group) (× 400)
图 6 过表达CeyPRDX1基因的鉴定. A: qRT-PCR检测CeyPRDX1的相对mRNA水平; B:Western-blot 检测CeyPRDX1-EGFP融合蛋白表达
Fig. 6 Identification of overexpression of CeyPRDX1 gene. A: qRT-PCR detection of relative mRNA levels of CeyPRDX1; B: Western blotting analysis for the expression of CeyPRDX1-EGFP fusion proteins
图 9 CeyPRDX1基因的RdN/RdS. Cey:塔里木马鹿;Ce:东欧马鹿;Cc:马鹿;Ovt:白尾鹿;Oa:绵羊;Ch:山羊;Od:弯角剑岭;Bm:牦牛;Bt:牛;Vp:羊驼;Cdr:单峰驼;Cb:双峰驼;Cf:野骆驼;Ss:野猪;Ea:驴;Ec:马;Eq:平原斑马;Ep:普氏野马;Cl:狼;Ua:棕熊;Am:大熊猫;Fc:猫;Pt:虎;Pl:狮子;Hs:人;Mm:猕猴;Rn:褐家鼠;Pmb:草原鹿鼠
Fig. 9 RdN/RdS of CeyPRDX1 gene. Cey: Cervus elaphus yarkandensis; Ce: Cervus elaphus; Cc: Cervus canadensis; Ovt: Odocoileus virginianus texanus; Oa: Ovis aries; Ch: Capra hircus; Od: Oryx dammah; Bm: Bos mutus; Bt: Bos taurus; Vp: Vicugna pacos; Cdr: Camelus dromedarius; Cb: Camelus bactrianus; Cf: Camelus ferus; Ss: Sus scrofa; Ea: Equus asinus; Ec: Equus caballus; Eq: Equus quagga; Ep: Equus przewalskii; Cl: Canis lupus; Ua: Ursus arctos; Am: Ailuropoda melanoleuca; Fc: Felis catus; Pt: Panthera tigris; Pl: Panthera leo; Hs: Homo sapiens; Mm: Macaca mulatta; Rn: Rattus norvegicus; Pmb: Peromyscus maniculatus bairdii
| 氨基酸 Amimo acid | 数目 Number count | 频率By frequency/% | 氨基酸 Amimo acid | 数目 Number count | 频率 By frequency/% | |
|---|---|---|---|---|---|---|
| 丙氨酸 Ala(A) | 15 | 6.2 | 赖氨酸 Lys(K) | 19 | 7.9 | |
| 精氨酸 Arg(R) | 17 | 7.0 | 甲硫氨酸 Met(M) | 4 | 1.7 | |
| 天冬酰胺 Asn(N) | 6 | 2.5 | 苯丙氨酸 Phe(F) | 15 | 6.2 | |
| 天冬氨酸 Asp(D) | 19 | 7.9 | 脯氨酸 Pro(P) | 13 | 5.4 | |
| 半胱氨酸 Cys(C) | 6 | 2.5 | 丝氨酸 Ser(S) | 21 | 8.7 | |
| 谷氨酰胺 Gln(Q) | 12 | 5.0 | 苏氨酸 Thr(T) | 11 | 4.5 | |
| 谷氨酸 Glu(E) | 10 | 4.1 | 色氨酸 Trp(W) | 2 | 0.8 | |
| 甘氨酸 Gly(G) | 18 | 7.4 | 酪氨酸 Tyr(Y) | 5 | 2.1 | |
| 组氨酸 His(H) | 4 | 1.7 | 缬氨酸 Val(V) | 14 | 5.8 | |
| 异亮氨酸 Ile(I) | 17 | 7.0 | 吡咯赖氨酸 Pyl(O) | 0 | 0.0 | |
| 亮氨酸Leu(L) | 14 | 5.8 | 硒代半胱氨酸 Sec(U) | 0 | 0.0 |
表2 CeyPRDX1基因氨基酸组成预测
Table 2 Predicted amino acid composition of the CeyPRDX1 gene
| 氨基酸 Amimo acid | 数目 Number count | 频率By frequency/% | 氨基酸 Amimo acid | 数目 Number count | 频率 By frequency/% | |
|---|---|---|---|---|---|---|
| 丙氨酸 Ala(A) | 15 | 6.2 | 赖氨酸 Lys(K) | 19 | 7.9 | |
| 精氨酸 Arg(R) | 17 | 7.0 | 甲硫氨酸 Met(M) | 4 | 1.7 | |
| 天冬酰胺 Asn(N) | 6 | 2.5 | 苯丙氨酸 Phe(F) | 15 | 6.2 | |
| 天冬氨酸 Asp(D) | 19 | 7.9 | 脯氨酸 Pro(P) | 13 | 5.4 | |
| 半胱氨酸 Cys(C) | 6 | 2.5 | 丝氨酸 Ser(S) | 21 | 8.7 | |
| 谷氨酰胺 Gln(Q) | 12 | 5.0 | 苏氨酸 Thr(T) | 11 | 4.5 | |
| 谷氨酸 Glu(E) | 10 | 4.1 | 色氨酸 Trp(W) | 2 | 0.8 | |
| 甘氨酸 Gly(G) | 18 | 7.4 | 酪氨酸 Tyr(Y) | 5 | 2.1 | |
| 组氨酸 His(H) | 4 | 1.7 | 缬氨酸 Val(V) | 14 | 5.8 | |
| 异亮氨酸 Ile(I) | 17 | 7.0 | 吡咯赖氨酸 Pyl(O) | 0 | 0.0 | |
| 亮氨酸Leu(L) | 14 | 5.8 | 硒代半胱氨酸 Sec(U) | 0 | 0.0 |
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