ACTA THERIOLOGICA SINICA ›› 2018, Vol. 38 ›› Issue (6): 562-571.DOI: 10.16829/j.slxb.150093

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Identification and Characterizations of Aeromonas salmonicida isolated from Yangtze finless porpoise

LIU Zhigang, JIANG Huyan, ZHANG Xiaoke, CHEN Minmin, WAN An,YU Daoping   

  1. (1College of Life Science, Anqing Normal University, Anqing 246011, China)
    (2 Research Center of Aquatic Organism conservation and Water Ecosystem Restoration in Anhui Province, Anqing Normal University, Anqing 246001, China)
  • Online:2018-11-30 Published:2018-11-26


刘志刚 蒋胡艳 张晓可 陈敏敏 万安 于道平   

  1. (1 安庆师范大学生命科学学院,安庆 246011)(2 水生生物保护与水生态修复安徽省高等学校工程技术研究中心,安庆 246011)
  • 通讯作者: 于道平


In this study, we have for the first time isolated Aeromonas salmonicida strain XJ-JT from Yangtze finless porpoise which showed clinical symptoms such as ulcers and rotten skin. We found that the strain XJ-JT was a gram-negative bacterium with blunt ends and non-sporulating; 16S rRNA gene fragments of of several strains were amplified and sequenced, and phylogenetic trees were constructed which reveal the similarity with Aeromonas salmonicida isolated from Silver carp. Moreover, we studied its biological characteristics by performing physicochemical characterization, genetic evolution analysis, drug susceptibility assays and a pathogenicity test. 20 common antibiotics were tested in drug susceptibility assays. We showed that XJ-JT is highly sensitive to cefotaxime sodium,  ceftriaxone sodium and kanamycin, moderately sensitive to cefotaxime sodium, ceftriaxone sodium and kanamycin, and not sensitive to amoxicillin, ampicillin, tetracycline, and so on. An in vivo infection assay showed that this new strain, XJ-JT, has a strong pathogenicity in Crucian carp.

Key words: Aeromonas salmonicida, Characterizations, Isolation and Identification, Yangtze finless porpoise



关键词: 长江江豚, 杀鲑气单胞菌, 分离鉴定, 生物学特性