ACTA THERIOLOGICA SINICA ›› 2017, Vol. 37 ›› Issue (4): 389-398.DOI: 10.16829/j.slxb.201704009

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Isolation and identification of intestinal Enterococcus faecalis from golden snub-nosed monkeys in Shennongjia National Natural Reserve

GUO Huihui,BAI Wenmei, LI Xiang, YANG Jingyuan, YAO Hui, XIAO Yuncai   

  1. State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University;Key Lab of Conservation Biology for Shennongjia Golden Monkey, Hubei Province
  • Online:2017-11-13 Published:2017-10-24


呙会会 白文妹 李翔 杨敬元 姚辉 肖运才   

  1. 华中农业大学动物医学院 农业微生物学国家重点实验室;湖北神农架金丝猴保育生物学湖北省重点实验室
  • 通讯作者: 肖运才 E-mail:
  • 基金资助:

Abstract: Eight strains with obvious antibacterial effect were isolated from the contents of the intestinal of healthy wild golden snub-nosed monkeys(Rhinopithcus roxellana) in Shennongjia by differential cultivation, morphology observation and antimicrobial tests. They were identificated as Enterococcus faecalis by physiological and biochemical identification, and 16S rRNA sequence analysis. Of which, two good safety strains were screened through the detection of virulence factors and the animal acute toxicity test. Their characteristics of tolerating gastrointestinal environment (low pH, high bile) and growth were examined. The results showed that E. faecalis dlt7a and dlt7b were safe, and probiotic for the body, and they own the features that rapid propagation, a strong ability to withstand the gastric acid and intestinal bile salt. They may be used as candidate probiotics strains for snub-nosed monkeys in the future.

Key words: Enterococcus faecalis, Isolation and identification, Probiotics, Golden snub-nosed monkeys

摘要: 通过培养特征、形态观察、抑菌性实验,从神农架健康野生金丝猴肠道分离到8株抑菌效果明显的菌株;生理生化鉴定和16S rRNA基因序列同源性分析,该8株菌为粪肠球菌。毒力因子检测和动物急性毒性实验筛选出2株安全性良好的菌株,研究两菌株对胃肠道环境(低pH值、高胆盐)的耐受特性和生长情况。结果表明,粪肠球菌dlt7a和dlt7b株繁殖能力很强,无迟缓期,具有较强的耐受胃酸及肠道高胆盐环境的能力,且安全性好,具有较好的益生特性,可作为金丝猴微生态制剂的候选菌株。

关键词: 金丝猴, 粪肠球菌, 分离鉴定, 益生菌