ACTA THERIOLOGICA SINICA ›› 2022, Vol. 42 ›› Issue (6): 677-686.DOI: 10.16829/j.slxb.150587

• ORIGINAL PAPERS • Previous Articles     Next Articles

The variations of nephrons and relevant function factors between hibernating and non-hibernating Daurian ground squirrels

Xiujing ZHANG1, Heng WANG1, Qiumei ZHONG1, Chenxi YANG1,2, Jianli WANG1,2()   

  1. 1.College of Biological Sciences and Engineering, North Minzu University, Yinchuan 750021, China
    2.Key Laboratory of Ecological Protection of Agro-Pastoral Ecotones in the Yellow River Basin of National Ethnic Affairs Commission, Yinchuan 750021, China
  • Received:2021-07-10 Accepted:2022-06-21 Online:2022-11-30 Published:2022-12-02
  • Contact: Jianli WANG


张修静1, 王恒1, 钟秋梅1, 杨晨希1,2, 王建礼1,2()   

  1. 1.北方民族大学生物科学与工程学院,银川 750021
    2.国家民委黄河流域农牧交错区生态保护重点实验室,银川 750021
  • 通讯作者: 王建礼
  • 作者简介:张修静 (2001- ),女,本科生,主要从事动物生理生态学研究;
    王恒 (1995- ),男,硕士研究生,主要从事动物生理生态学研究;第一联系人:.共同第一作者
  • 基金资助:


Hibernation is an adaptive survival strategy through which the hibernating species copes with months of low ambient temperature and a lack of food. The Daurian ground squirrel (Spermophilus dauricus) is a typical fat-storing hibernator. To further investigate the renal adaptive mechanisms of hibernators, the nephronal morphology changes and the concentrations of serum creatinine, urine and antidiuretic hormone (ADH) were examined during summer active (July), winter hibernating (December), and early spring arousal (March) periods in the Daurian ground squirrel by the means of histology, blood biochemical analysis, and enzyme-linked immunosorbent assay. The gene expression of aquaporins (AQP1, AQP2,and AQP3), ADH receptor (V2R), and endothelial nitric oxide synthase (eNOS) were also examined by the means of real-time PCR. The density of glomerulus, relative diameter of distal tubules and proximal tubules, and ratio of corticomedullary proximal tubules to distal tubules were found to be lower during hibernating and arousal periods compared to active period. The concentration of serum creatinine and urine increased significantly during hibernating period compared to those during active and arousal periods. The serum ADH concentration and V2R gene expression level decreased significantly during hibernating period compared to those during active periods. The gene expression of AQP1 during hibernating period was higher than that during arousal period, and the AQP3 gene expression was lower than that during active period, while the AQP2 gene expression level did not change significantly. The eNOS gene expression during hibernating period was lower than that during arousal period. These results indicate that the Daurian ground squirrel lowered its renal function during hibernation. The aquaporins, eNOS, and ADH show functional adjustments to adapt to different periods. These results might provide a better understanding of the renal adaptation mechanism in hibernating animals.

Key words: Daurian ground squirrel, Hibernation, Kidney, Aquaporins, Antidiuretic hormone


冬眠是动物应对冬季低温和食物匮乏的一种生存策略。达乌尔黄鼠 (Spermophilus dauricus) 是典型的贮脂类冬眠动物。为研究冬眠动物肾脏的适应机制,本实验采用组织学、血液生化分析及酶联免疫方法检测了夏季活动期 (7月) 、冬眠期 (12月) 和早春出眠后 (3月) 达乌尔黄鼠肾单位形态学及血清肌酐、尿素和抗利尿激素 (ADH) 的变化,并用qPCR方法检测了肾脏水通道蛋白基因 (AQP1AQP2AQP3)、ADH受体 (V2R) 及内皮型一氧化氮合酶基因 (eNOS) 的表达。结果发现,冬眠期和早春出眠期的达乌尔黄鼠肾小球密度、近曲小管和远曲小管的相对管径、皮质部近曲小管数与远曲小管数比值均低于夏季活动期;冬眠期血清肌酐和尿素浓度高于夏季活动期和早春出眠期,ADH浓度及其受体V2R基因表达低于夏季活动期;冬眠期AQP1基因表达高于早春出眠期,AQP3基因表达低于夏季活动期,AQP2基因表达无显著差异;冬眠期eNOS基因表达低于早春出眠期。这些结果表明冬眠的达乌尔黄鼠表现出较低的肾功能;不同时期的水通道蛋白,eNOS及ADH表现出适应性的功能调节。该实验结果丰富了对冬眠动物肾脏适应机制的认识。

关键词: 达乌尔黄鼠, 冬眠, 肾脏, 水通道蛋白, 抗利尿激素

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