Construction and sequence analysis of a cDNA library from the liver of Microtus fortis

Abstract

Abstract: The plasmid cDNA library from the liver of Microtus fortis was constructed by using SMART technology. The titers of this cDNA library were 1076 pfu / μL,the recombination rate was about 94%,the library capacity was 1.08 × 10⁶ . 22 cDNAs chosen at random were sequenced. After BLAST analysis of these sequences,it was found that these cDNAs showed more than 75% similarity to the functional known genes of mice,rats and humans in the Genbank. The cDNA library was very important to the research of fatty liver-related gene screening and clones. These provided a base for further study on the molecular biology,fatty liver model development and pathogenesis research of Microtus fortis.

Key words: cDNA library, Liver, Microtus fortis

关键词: cDNA 文库, 东方田鼠, 肝脏

FENG Jie,XIE Jianyun,SUN Qiang,YANG Yuqin,HU Jianhua,GAO Cheng. Construction and sequence analysis of a cDNA library from the liver of Microtus fortis[J]. .

冯洁,谢建云,孙强,杨玉琴,胡建华,高诚. 东方田鼠肝脏cDNA 文库的构建及部分克隆序列分析[J]. .

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Construction and sequence analysis of a cDNA library from the liver of Microtus fortis

东方田鼠肝脏cDNA 文库的构建及部分克隆序列分析

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