Abstract: Tannins are phenolic secondary metabolites that are present in various plants and have potential antioxidant properties. However, the effect of tannins on autophagy in animals remains unclear. In the present study, 4-week-old Brandt's voles (Lasiopodomys brandtii) were fed low and high doses of tannic acid (TA) solution for 9 weeks. The variations in serum antioxidant levels and the expression of liver autophagy-related genes in relation to the TA dose were determined by enzyme-linked immunosorbent assay (ELISA) and fluorescent quantitative polymerase chain reaction (qPCR), respectively. TA had no evident effects on the rates of growth of body weight and liver index in the voles. The level of serum superoxide dismutase (SOD) was higher in the high-dose TA group than in the control group, but the level of serum malondialdehyde (MDA) and the expression levels of both Beclin1 and p62 in the liver were lower in the highdose TA group than in the control group. Levels of serum glutathione peroxidase (GSH-Px) and catalase (CAT) were higher in the high-dose TA group than in the control group, but the expression level of LC3A in the liver was lower in the high-dose group than in the control group. In both the low-dose and high-dose TA groups, the serum GSH-Px levels were higher in male voles than in female voles, and in the high-dose TA group, the serum CAT levels were also higher in male voles than in female voles. Our research demonstrates that certain doses of TA could improve antioxidant levels and reduce autophagy in the liver, but the response of the antioxidant system to TA was stronger in male voles than in female voles. Our findings suggest that low consumption of tannin from plants could improve the antioxidant capabilities of wild Brandt's voles, thereby benefiting their population.

Key words: Brandt's vole, Tannic acid, Antioxidant, Autophagy, Liver, Genes expression

摘要： 单宁为植物中广泛存在的酚类植物次生代谢物，具有抗氧化功能，但是否影响动物细胞的自噬功能，仍不清楚。本研究对4周龄雌雄性布氏田鼠饲喂高、低浓度单宁酸溶液9周后，分别用酶联免疫吸附法和荧光定量PCR法测定血浆抗氧化水平和肝脏细胞自噬相关基因表达的变化。结果显示，单宁酸对布氏田鼠的体重增长率和肝脏指数均无显著影响。高剂量组布氏田鼠血浆超氧化物歧化酶（SOD）水平显著高于对照组，而血浆丙二醛（MDA）水平、肝脏自噬相关基因Beclin1和p62的表达量都显著低于对照组。在雄性布氏田鼠中，高剂量组血浆谷胱甘肽过氧化物酶（GSH-Px）和过氧化氢酶（CAT）水平都显著高于对照组，肝脏自噬相关基因LC3A表达量显著低于对照组。低剂量组和高剂量组雄鼠血浆的GSH-Px水平，以及高剂量组雄鼠血浆的CAT水平都高于雌性布氏田鼠。本研究表明一定剂量的单宁酸能提高布氏田鼠的抗氧化水平，降低肝脏细胞自噬，同时雄性布氏田鼠抗氧化系统对单宁酸的响应程度大于雌性布氏田鼠，提示低量植物单宁摄入将提高野生布氏田鼠的抗氧化能力而有利于其种群。

关键词: 布氏田鼠, 单宁, 抗氧化, 自噬, 肝脏, 基因表达