Abstract: To reexamine genetic divergence of Korean Crocidura lasiura from an adjacent far-eastern Russian population, we obtained Cyt b complete nine sequences (1 140 bp)of C. lasiura from four locations in Korea and three locations in fareastern Russia,and these sequences were compared to the corresponding complete four and partial (402 bp)four sequences of C. lasiura,obtained from GenBank. We found from this Cyt b complete sequence analysis that Korean C. lasiura is not monogenic,and concluded that Cyt b partial sequences of C. lasiura have to be used with caution for population structure investigation. More importantly,we detected from this analysis with Cyt b complete sequences that Korean C. lasiura is distinct from other C. lasiura in far-eastern Russia,with average JC distance of 1. 08% and seven fixed site differences,and consider that genetically divergent Korean C. lasiura has not been in contact from other C. lasiura in adjacent far-eastern Russia even during the last glacial maxima. Finally,we found that present sequencing results do not support the current
subspecies classification,recognizing C. lasiura as a monotypic species,but do support a previous classification,recognized Korean C. lasiura as C. l. thomasi,although further sequencing analyses with more C. lasiura specimens from northeastern China are needed to confirm the present findings

Key words: Biogeography, Crocidura lasiura, Cyt b gene, DNA ststematics, Far-eastern Russia, Korea

摘要： 为重新分析韩国大麝鼩种群与相邻的俄罗斯远东种群之间的遗传分化情况,我们获得9 条来自韩国4 个地点和俄罗斯3 个地点的大麝鼩线粒体细胞色素b 基因(Cyt b)全序列,并将其与来自GenBank 的4 条Cyt b 全序列和4 条Cyt b 部分序列进行比较。结果发现韩国的大麝鼩并非只有一种基因型,因此,在利用Cyt b 部分序列进行种群遗传学分析时需格外注意。基于Cyt b 全序列分析,发现韩国的大麝鼩与俄罗斯远东地区的大麝鼩之间存在1.08% 的平均JC 距离和7 个位点的差异,推测韩国的大麝鼩与俄罗斯远东地区的大麝鼩在包括末次冰盛期在内的很长时期都没有进行过遗传交流。目前的测序结果不支持当前认为大麝鼩为单系群的亚种分类理论,支持韩国大麝鼩是C. l. thomasi 亚种的分类理论,但还需进一步对中国东北地区的样品进行测序分析后才能最终确认。

关键词: 生物地理学, DNA 系统分类学, Cyt b 基因, 大麝鼩, 韩国, 俄罗斯远东地区