Abstract: Rousettus leschenaulti is mainly distributed in tropical and semi-tropical areas. Populations are large and this species eats fruits,such as longan and litchi. In China,it has been subjected to large-scale killing and the population size has decreased dramatically. In order to study its population structure and genetic diversity,we constructed an enriched DNA microsatellite library of this species,and obtained four pairs of special polymorphism microsatellite primers. Moreover, we examined the applicability of 10 microsatellite markers of Rousettus leschenaulti in four closely related species (Cynopterus brachyotis, Cynopterus sphinx, Eonycteris spelaea, Macroglossus minimus).

Key words: Applicability, Closely related species, Microsatellite, Rousettus leschenaulti

摘要： 棕果蝠在我国热带和亚热带地区分布广、种群数量大,对龙眼、荔枝等水果种植业造成巨大危害。近年来,棕果蝠在国内被大范围捕杀,造成其种群数量急剧下降。为了获得用于研究棕果蝠的种群结构和遗传多样性的分子标记,我们利用探针富集法构建了棕果蝠部分微卫星基因组文库,筛选出棕果蝠的4 个高度多态性位点;并结合前期本研究组已经发表的棕果蝠微卫星位点,共检测了10 个棕果蝠特异的微卫星标记在其它4 种果蝠(短耳犬蝠、犬蝠、大长舌果蝠、小长舌果蝠)内的通用性,为不同果蝠种群遗传学研究提供了更多的微卫星分子标记。

关键词: 棕果蝠, 微卫星, 近缘种, 通用性