ACTA THERIOLOGICA SINICA ›› 2021, Vol. 41 ›› Issue (3): 284-295.DOI: 10.16829/j.slxb.150485

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Research advances of the host DNA enrichment from noninvasively collected samples

WANG Liling, HU Jingyang, KUANG Weimin, YU Li   

  1. School of Life Sciences of Yunnan University, State Key Laboratory for Conservation and Utilization of Bio-Resource in Yunnan, Kunming 650091, China
  • Received:2020-09-10 Online:2021-05-30 Published:2021-05-27


王李玲, 胡靖扬, 匡卫民, 于黎   

  1. 云南大学生命科学学院, 省部共建云南生物资源保护与利用国家重点实验室, 昆明 650091
  • 通讯作者: 于黎,
  • 作者简介:王李玲(1995-),女,在读硕士研究生,主要从事遗传学研究.
  • 基金资助:

Abstract: Noninvasive sampling has been widely used in conservation genetics, molecular ecology and molecular evolution study of animals. With the development of genome sequencing and the advent of the genomics era, how to obtain high quality DNA used for genome sequencing from noninvasively collected samples is a challenge for researchers. In this review, we summarized and compared the methods and applications of enriching host DNA in fecal samples and archaeological materials or museum specimens, which were the most common used sample sources in the noninvasive sampling. We aimed to provide an important reference for the future research and application of noninvasive sampling in the genomic study of the animals.

Key words: Noninvasive sampling, Enrichment host DNA, Fecal DNA, Ancient DNA, Hybridization capture, MBD enrichment

摘要: 非损伤性取样被广泛应用在动物保护遗传学、分子生态学和分子进化等研究领域。随着基因组测序技术的发展和基因组学时代的到来,如何从非损伤性取样样品中获取能够用于进行基因组测序的高质量DNA是研究者面临的难题。本文总结和比较了非损伤性取样中最常用的粪便样品和考古材料或博物馆标本两类样品中富集宿主DNA的方法及应用,以期为非损伤性取样在动物基因组学的研究和应用提供重要参考。

关键词: 非损伤性取样, 宿主DNA富集, 粪便DNA, 古DNA, 杂交捕获, MBD富集

CLC Number: