ACTA THERIOLOGICA SINICA ›› 2020, Vol. 40 ›› Issue (5): 458-466.DOI: 10.16829/j.slxb.150341

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Genetic diversity and structure of the Alashan red deer (Cervus elaphus alashanicus) in Helan Mountains, China

GAO Hui1,QIAO Fujie2,TENG Liwei 1,4,LI Junle1,YU Mengqi 3,LIU Zhensheng 1,4#br#   

  1. (1 College of Wildlife and Protected Area, Northeast Forestry University, Harbin 150040, China)
    (2 School of Life Sciences, Lüliang Univiersity, Lüliang 033000, China)
    (3 Forest Pest Control and Quarantine Station of Ningxia Hui Autonomous Region, Yinchuan 750021, China)
    (4 Key Laboratory of Conservation Biology, State Forestry and Grassland Administration, Harbin 150040, China)
  • Online:2020-09-30 Published:2020-09-27


高惠1  乔付杰2  滕丽微1,4  李俊乐1  余梦琦3  刘振生1,4   

  1. (1东北林业大学野生动物与自然保护地学院,哈尔滨 150040)(2吕梁学院生命科学系,吕梁 033000)(3宁夏回族自治区森林病虫防治检疫总站,银川 750021)(4国家林业和草原局野生动物保护学重点开放实验室,哈尔滨 150040)
  • 通讯作者: 刘振生
  • 基金资助:

Abstract: Alashan red deer (Cervus elaphus alashanicus) is only found in Helan Mountainous areas. It is an isolated population, and has the narrowest distribution range among the eight red deer subspecies in China. Up to now little is known about its genetics. To understand its genetic diversity and variation, and to provide scientific reference for amending its conservation strategies, partial sequences of mitochondrial control region (991 bp), taken from fresh feces samples of 93 wild individuals from Helan Mountains, were amplified and analyzed. The results indicate that, based on 68 polymorphic sites and defined 16 haplotypes, genetic diversity for this species is low; its mean haplotype diversity and nucleotide diversity are only 0.405 and 0.00232, respectively. The analysis of Mismatch distributions and neutral tests demonstrates that the Alashan red deer has experienced a population expansion, and further Bayesin skyline plot (BSP) implies that such an expansion may have occurred during the Last Glacial Maximum (0.028-0.010Ma). The FST test shows that genetic variation of the deer is prominent. The phylogenetic tree constructed with Bayesian method and haplotype network indicates that there is no obvious phylogeographic separation between the two geographic groups. The final conclusion of this study indicates that genetic diversity of the Alashan red deer studied is quite low. Thus, more attentions to increasing its genetic exchanges with  other geographic groups or subspecies is critically required in order to maintain its prosperous prospect.

Key words: Alashan red deer(Cervus elaphus alashanicus), Fecal DNA, MtDNA D-loop, Genetic diversity, Genetic differentiation


马鹿阿拉善亚种(Cervus elaphus alashanicus)又称阿拉善马鹿,目前仅分布于贺兰山地区,是我国马鹿亚种分布范围最狭窄的一个隔离种群。为了解阿拉善马鹿的种群遗传多样性及遗传变异情况,以对该种群的保护提供科学参考,对在贺兰山采集的93个野生个体新鲜粪便样本的线粒体控制区部分序列(991 bp)进行扩增和分析,共检测到68个变异位点,定义16种单倍型,平均单倍型多样性为0.405,平均核苷酸多样性为0.00232,说明种群遗传多样性水平较低。中性检验和错配分布分析表明阿拉善马鹿曾出现过种群扩张,贝叶斯天际线分析(BSP)显示扩张时间约在末次冰盛期(0.028—0.010 Ma)。FST检验表明阿拉善马鹿种群内存在显著遗传分化,系统发育树和单倍型网络图分析表明群体间没有明显的系统地理格局。本研究表明阿拉善马鹿目前种群遗传多样性较低,建议加大对该亚种的关注和保护力度。

关键词: 阿拉善马鹿, 粪便DNA, 线粒体控制区, 遗传多样性, 遗传分化