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超氧化物歧化酶同工酶分析法用于兽类分类的试探

何新霞, 周虞灿, 邵邻相   

  1. 浙江师范大学生物系
  • 出版日期:2006-07-29 发布日期:2007-09-13

A TEST ON THE WAY OF SUPER0XIDE DISMUTASE ISOZYMES TO MAMMALLAN TAXONOMY

HE Xinxia, ZHOU Yucan, SHAO Lingxiang   

  • Online:2006-07-29 Published:2007-09-13

摘要: 本文以蝙蝠的5种组织和鼠类的红细胞为材料,从方法上探讨SOD同工酶的分析比较对于兽类分类的价值和意义。实验结果表明SOD同工酶的泳动速度和区带的分布组成, 种与种之间有明显的区别,作为一个辅助的工具酶对于兽类的演化和物种的鉴定比较适用。此外,同种的个体间以及同个体的组织间未发现明显区别,这预示在分类工作中不必强调被分析个体数的多寡,也不必进行各组织的全面分析,有利于取材不易的或不能杀死取材时珍稀兽类物种间的比较分析。

关键词: 蝙蝠, 鼠类, SOD同工酶

Abstract: This paper is to try to find out the significance and effect to apply the analytic
method of SOD isozymes to mammalian taxonomy by polyacrylamide gel eloctrophoresisof the isozymes in the tissues of bats and the erythrocytes of murids. The obtainedresults show that SOD isozyme is a veryuseful tool enzyme to distinguish new speciesor evolution of mammal,because there are marked differences of the mobility, thecomposition and the distribution of SOD isozymes in different species.But there are notnotable differences about above-mentioned characteristics of SOD isozyrnes in differentindividual of same species and in different tissue of same individual.The resultssuggest that it would be not necessity to emphasize the number of animal analysed andnot necessity to analyse every tissues. It is very good to make research on proteinbiochemical types of rare mammals,because they are very scarce and not killed in order
to draw materials.

Key words: Bats, murids, SOD isozyme