Research progress of interspecies somatic cell nuclear transfer in wild animals

doi:10.16829/j.slxb.150642

Abstract

Abstract:

Interspecies somatic cell nuclear transfer (iSCNT) is one of the most promising research directions in somatic cell nuclear transfer (SCNT). It has a wide application in the production of transgenic animals, the breeding of improved varieties, the protection of endangered animals, the regeneration of extinct species, as well as the breeding of wildlife and the protection of biodiversity. In this article, we review the research progress and technical barriers of interspecific nuclear transfer technology in cats, canines, bovids, swine and peccaries, and provide theoretical and technical guidance for further improvement of utilization of iSCNT technology in wild animal reproduction, breeding and diversity protection.

Key words: Wild animals, Somatic cell, Interspecies nuclear transfer, Diversity

摘要：

种间体细胞核移植 (Interspecies somatic cell nuclear transfer, iSCNT) 作为体细胞核移植 (Somatic cell nuclear transfer, SCNT) 中极具潜力的研究方向之一，在转基因动物的生产、优良种群的繁育、濒危动物的保护、灭绝物种的“再生”以及野生动物繁育和生物多样性保护等方面具有广泛的应用前景。本文通过对猫科、犬科、牛科、猪科和西貒科等野生动物的种间核移植技术的研究进展和技术屏障进行阐述，为促进iSCNT技术在野生动物繁殖、育种和多样性保护等方面的进一步应用提供理论基础和技术指导。

关键词: 野生动物, 体细胞, 种间核移植, 多样性

CLC Number:

Shigang GU, Yanhui ZHAI, Chao XU, Dawei YU, Zhiqiang HAN, Quanmin ZHAO, Xiangpeng DAI. Research progress of interspecies somatic cell nuclear transfer in wild animals[J]. ACTA THERIOLOGICA SINICA, 2022, 42(4): 442-450.

顾士钢, 翟岩辉, 徐超, 余大为, 韩志强, 赵全民, 代相鹏. 野生动物种间体细胞核移植研究进展[J]. 兽类学报, 2022, 42(4): 442-450.

Figures/Tables 4

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辅助生殖技术 Assisted reproductive technology	首次应用 First application	后续发展 Follow up development
体外受精 In vitro fertilization	1890年兔 In 1890， rabbit	1949年绵羊、山羊 In 1949， sheep， goat 1951年猪、牛 In 1951， pig， cattle 1974年牛 In 1974，cattle
人工授精 Artificial insemination	1780年犬 In 1780， dog	1912年马、牛和羊 In 1912， horse， cattle and sheep 1952年牛 In 1952， cattle 1961年猪 In 1961， pig
体外胚胎生产 In vitro embryo production	1987年牛 In 1987， cattle	1991年水牛 In 1991， buffalo 2000年猪 In 2000， pig 2003年马 In 2003， horse
体细胞核移植 Somatic cell nuclear transfer	1997年绵羊 In 1997， sheep	1998年鼠 In 1998， mouse 1999年水牛 In 1999， buffalo 2000年猪 In 2000， pig 2001年山羊 In 2001， goat 2002年兔 In 2002， rabbit 2002年猫 In 2002， cat 2003年骡子 In 2003， mule 2003年马 In 2003， horse
超数排卵 Superovulation techniques	1972年鼠 In 1972， mouse	1983年山羊 In 1983， goat 1993年犬 In 1993， dog 1997年兔 In 1997， rabbit 2002年猫 In 2002， cat

辅助生殖技术 Assisted reproductive technology	首次应用 First application	后续发展 Follow up development
体外受精 In vitro fertilization	1890年兔 In 1890， rabbit	1949年绵羊、山羊 In 1949， sheep， goat 1951年猪、牛 In 1951， pig， cattle 1974年牛 In 1974，cattle
人工授精 Artificial insemination	1780年犬 In 1780， dog	1912年马、牛和羊 In 1912， horse， cattle and sheep 1952年牛 In 1952， cattle 1961年猪 In 1961， pig
体外胚胎生产 In vitro embryo production	1987年牛 In 1987， cattle	1991年水牛 In 1991， buffalo 2000年猪 In 2000， pig 2003年马 In 2003， horse
体细胞核移植 Somatic cell nuclear transfer	1997年绵羊 In 1997， sheep	1998年鼠 In 1998， mouse 1999年水牛 In 1999， buffalo 2000年猪 In 2000， pig 2001年山羊 In 2001， goat 2002年兔 In 2002， rabbit 2002年猫 In 2002， cat 2003年骡子 In 2003， mule 2003年马 In 2003， horse
超数排卵 Superovulation techniques	1972年鼠 In 1972， mouse	1983年山羊 In 1983， goat 1993年犬 In 1993， dog 1997年兔 In 1997， rabbit 2002年猫 In 2002， cat

物种 Name of species	IUCN 类别 IUCN Category	国家 Country	供核细胞 Karyoplast	供卵母细胞动物 Oocyte donor	卵裂率 Cleavage rate（%）	桑椹胚率 Morula percentage（%）	囊胚率 Blastocyst percentage（%）	妊娠率 Pregnancy rate（%）	产仔数 No. of births （only）	文献 References
虎 Panthera tigris	EN	意大利 Italy	成纤维细胞 Fibroblasts	兔 Rabbit	N/A	19.8	4.9	N/A	—	Yelisetti et al.， 2016
虎 Panthera tigris	EN	阿根廷 Argentina	成纤维细胞 Fibroblasts	家猫 Domestic cat	N/A	18.6	3.4	N/A	—	Moro et al.， 2015
虎 Panthera tigris	EN	阿根廷 Argentina	成纤维细胞 Fibroblasts	家猫 Domestic cat	N/A	25.4	12.7	N/A	—	Moro et al.， 2015
狮子 Panthera leo	EN	意大利 Italy	成纤维细胞Fibroblasts	兔 Rabbit	N/A	13.5	4.5	N/A	—	Yelisetti et al.， 2016
亚洲猎豹 Acinonyx jubatus venaticus	VU	伊朗 Iran	成纤维细胞Fibroblasts	家猫 Domestic cat	N/A	5.9	—	—	—	Moulavi et al.， 2017
花豹 Panthera pardus	VU	意大利 Italy	成纤维细胞Fibroblasts	兔 Rabbit	N/A	13.4	4.1	N/A	—	Yelisetti et al.， 2016
猎豹 Acinonyx jubatus	VU	阿根廷 Argentina	成纤维细胞Fibroblasts	家猫 Domestic cat	N/A	43.7	37.4	N/A	—	Moro et al.， 2015
猎豹 Acinonyx jubatus	VU	阿根廷 Argentina	成纤维细胞Fibroblasts	家猫 Domestic cat	N/A	37.8	22.3	N/A	—	Moro et al.， 2015
豹猫 Prionailurus bengalensis	LC	阿根廷 Argentina	成纤维细胞Fibroblasts	家猫 Domestic cat	N/A	38.0	37.2	N/A	—	Thongphakdee et al.， 2010
豹猫 Prionailurus bengalensis	LC	阿根廷 Argentina	成纤维细胞Fibroblasts	家猫 Domestic cat	N/A	35.1	46.9	N/A	—	Thongphakdee et al.， 2010
狞猫 Caracal caracal	LC	美国 USA	成纤维细胞Fibroblasts	猫 Cat	N/A	N/A	N/A	N/A	1	Pope， 2014
非洲野犬 Lycaon pictus	EN	韩国 Korea	成纤维细胞 Fibroblasts	犬 Dog	81.9	N/A	N/A	3.4	—	Son et al.， 2021
郊狼 Canis latrans	LC	韩国 Korea	胎儿成纤维细胞 Fetal Fibroblasts	家犬 Domestic dog	87.3	N/A	N/A	17.6	5	Hwang et al.， 2013
郊狼 Canis latrans	LC	韩国 Korea	成纤维细胞 Fibroblasts	家犬 Domestic dog	85.7	N/A	N/A	60.0	3	Hwang et al.， 2013
貉 Nyctereutes procyonoides	LC	韩国 Korea	成纤维细胞 Fibroblasts	猪 Pig	77.5	N/A	N/A	—	—	Jeon et al.， 2016
印度野牛 Bos gaurus	VU	泰国 Thailand	成纤维细胞 Fibroblasts	牛 Bovine	N/A	46.3	35.3	25.0	1	Srirattana et al.， 2012
印度野牛 Bos gaurus	VU	泰国 Thailand	成纤维细胞 Fibroblasts	牛 Bovine	N/A	45.0	35.2	26.0	—	Srirattana et al.， 2012
野水牛 Bubalus arnee	EN	印度 India	成纤维细胞 Fibroblasts	水牛 Buffalo	93.2	N/A	38.0	—	—	Priya et al.， 2014
野水牛 Bubalus arnee	EN	印度 India	成纤维细胞 Fibroblasts	水牛 Buffalo	93.4	N/A	53.9	—	—	Saini et al.， 2015
野水牛 Bubalus arnee	EN	印度 India	上皮细胞 Epithelial cell	水牛 Buffalo	85.4	N/A	20.2	—	—	Saini et al.， 2015
盘羊 Ovis ammon	NT	中国 China	成纤维细胞 Fibroblasts	羊 Sheep	84.3	N/A	15.7	—	—	Pan et al.， 2014

物种 Name of species	IUCN 类别 IUCN Category	国家 Country	供核细胞 Karyoplast	供卵母细胞动物 Oocyte donor	卵裂率 Cleavage rate（%）	桑椹胚率 Morula percentage（%）	囊胚率 Blastocyst percentage（%）	妊娠率 Pregnancy rate（%）	产仔数 No. of births （only）	文献 References
虎 Panthera tigris	EN	意大利 Italy	成纤维细胞 Fibroblasts	兔 Rabbit	N/A	19.8	4.9	N/A	—	Yelisetti et al.， 2016
虎 Panthera tigris	EN	阿根廷 Argentina	成纤维细胞 Fibroblasts	家猫 Domestic cat	N/A	18.6	3.4	N/A	—	Moro et al.， 2015
虎 Panthera tigris	EN	阿根廷 Argentina	成纤维细胞 Fibroblasts	家猫 Domestic cat	N/A	25.4	12.7	N/A	—	Moro et al.， 2015
狮子 Panthera leo	EN	意大利 Italy	成纤维细胞Fibroblasts	兔 Rabbit	N/A	13.5	4.5	N/A	—	Yelisetti et al.， 2016
亚洲猎豹 Acinonyx jubatus venaticus	VU	伊朗 Iran	成纤维细胞Fibroblasts	家猫 Domestic cat	N/A	5.9	—	—	—	Moulavi et al.， 2017
花豹 Panthera pardus	VU	意大利 Italy	成纤维细胞Fibroblasts	兔 Rabbit	N/A	13.4	4.1	N/A	—	Yelisetti et al.， 2016
猎豹 Acinonyx jubatus	VU	阿根廷 Argentina	成纤维细胞Fibroblasts	家猫 Domestic cat	N/A	43.7	37.4	N/A	—	Moro et al.， 2015
猎豹 Acinonyx jubatus	VU	阿根廷 Argentina	成纤维细胞Fibroblasts	家猫 Domestic cat	N/A	37.8	22.3	N/A	—	Moro et al.， 2015
豹猫 Prionailurus bengalensis	LC	阿根廷 Argentina	成纤维细胞Fibroblasts	家猫 Domestic cat	N/A	38.0	37.2	N/A	—	Thongphakdee et al.， 2010
豹猫 Prionailurus bengalensis	LC	阿根廷 Argentina	成纤维细胞Fibroblasts	家猫 Domestic cat	N/A	35.1	46.9	N/A	—	Thongphakdee et al.， 2010
狞猫 Caracal caracal	LC	美国 USA	成纤维细胞Fibroblasts	猫 Cat	N/A	N/A	N/A	N/A	1	Pope， 2014
非洲野犬 Lycaon pictus	EN	韩国 Korea	成纤维细胞 Fibroblasts	犬 Dog	81.9	N/A	N/A	3.4	—	Son et al.， 2021
郊狼 Canis latrans	LC	韩国 Korea	胎儿成纤维细胞 Fetal Fibroblasts	家犬 Domestic dog	87.3	N/A	N/A	17.6	5	Hwang et al.， 2013
郊狼 Canis latrans	LC	韩国 Korea	成纤维细胞 Fibroblasts	家犬 Domestic dog	85.7	N/A	N/A	60.0	3	Hwang et al.， 2013
貉 Nyctereutes procyonoides	LC	韩国 Korea	成纤维细胞 Fibroblasts	猪 Pig	77.5	N/A	N/A	—	—	Jeon et al.， 2016
印度野牛 Bos gaurus	VU	泰国 Thailand	成纤维细胞 Fibroblasts	牛 Bovine	N/A	46.3	35.3	25.0	1	Srirattana et al.， 2012
印度野牛 Bos gaurus	VU	泰国 Thailand	成纤维细胞 Fibroblasts	牛 Bovine	N/A	45.0	35.2	26.0	—	Srirattana et al.， 2012
野水牛 Bubalus arnee	EN	印度 India	成纤维细胞 Fibroblasts	水牛 Buffalo	93.2	N/A	38.0	—	—	Priya et al.， 2014
野水牛 Bubalus arnee	EN	印度 India	成纤维细胞 Fibroblasts	水牛 Buffalo	93.4	N/A	53.9	—	—	Saini et al.， 2015
野水牛 Bubalus arnee	EN	印度 India	上皮细胞 Epithelial cell	水牛 Buffalo	85.4	N/A	20.2	—	—	Saini et al.， 2015
盘羊 Ovis ammon	NT	中国 China	成纤维细胞 Fibroblasts	羊 Sheep	84.3	N/A	15.7	—	—	Pan et al.， 2014

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