ACTA THERIOLOGICA SINICA ›› 2023, Vol. 43 ›› Issue (3): 304-314.DOI: 10.16829/j.slxb.150671
• ORIGINAL PAPERS • Previous Articles Next Articles
Shuzhen ZOU1,2, Ya LUO1,2, Ming CHENG1,2, Fan WANG1,2, Dayong LI1,2, Di KANG1,2, Yun TANG1,2()
Received:
2022-03-24
Accepted:
2022-10-19
Online:
2023-05-31
Published:
2023-05-18
Contact:
Yun TANG
邹书珍1,2, 罗亚1,2, 程鸣1,2, 王凡1,2, 黎大勇1,2, 康迪1,2, 唐贇1,2()
通讯作者:
唐贇
作者简介:
邹书珍 (1990- ),女,副教授,博士,主要从事微生物生态方面的研究.
基金资助:
CLC Number:
Shuzhen ZOU, Ya LUO, Ming CHENG, Fan WANG, Dayong LI, Di KANG, Yun TANG. Characteristics of tetracycline antibiotic resistance genes in intestinal microorganisms and intestinal environment of Yunnan snub-nosed monkeys[J]. ACTA THERIOLOGICA SINICA, 2023, 43(3): 304-314.
邹书珍, 罗亚, 程鸣, 王凡, 黎大勇, 康迪, 唐贇. 滇金丝猴肠道微生物四环素抗性基因和肠道环境特征[J]. 兽类学报, 2023, 43(3): 304-314.
基因名称 Gene name | 上游引物 Forward primer | 下游引物 Reverse primer |
---|---|---|
tet (B) | AGTGCGCTTTGGATGCTGTA | AGCCCCAGTAGCTCCTGTGA |
tet (C) | CATATCGCAATACATGCGAAAAA | AAAGCCGCGGTAAATAGCAA |
tet (O) | ATGTGGATACTACAACGCATGAGATT | TGCCTCCACATGATATTTTTCCT |
tet (X) | AAATTTGTTACCGACACGGAAGTT | CATAGCTGAAAAAATCCAGGACAGTT |
tet (A) | GCTGTTTGTTCTGCCGGAAA | GGTTAAGTTCCTTGAACGCAAACT |
tet (D) | TGCCGCGTTTGATTACACA | CACCAGTGATCCCGGAGATAA |
tet (E) | TTGGCGCTGTATGCAATGAT | CGACGACCTATGCGATCTGA |
tet (M) | CATCATAGACACGCCAGGACATAT | CGCCATCTTTTGCAGAAATCA |
intI | GGCATCCAAGCAGCAAG | AAGCAGACTTGACCTGA |
intI2 | CAC GGA TAT GCG ACA AAA AGG T | GTA GCA AAC GAG TGA CGA AAT G |
intI1 | CGAACGAGTGGCGGAGGGTG | TACCCGAGAGCTTGGCACCCA |
IS613 | AGGTTCGGACTCAATGCAACA | TTCAGCACATACCGCCTTGAT |
tnpA-01 | CATCATCGGACGGACAGAATT | GTCGGAGATGTGGGTGTAGAAAGT |
tnpA-02 | GGGCGGGTCGATTGAAA | GTGGGCGGGATCTGCTT |
tnpA-03 | AATTGATGCGGACGGCTTAA | TCACCAAACTGTTTATGGAGTCGTT |
Table 1 Primer list used in this study
基因名称 Gene name | 上游引物 Forward primer | 下游引物 Reverse primer |
---|---|---|
tet (B) | AGTGCGCTTTGGATGCTGTA | AGCCCCAGTAGCTCCTGTGA |
tet (C) | CATATCGCAATACATGCGAAAAA | AAAGCCGCGGTAAATAGCAA |
tet (O) | ATGTGGATACTACAACGCATGAGATT | TGCCTCCACATGATATTTTTCCT |
tet (X) | AAATTTGTTACCGACACGGAAGTT | CATAGCTGAAAAAATCCAGGACAGTT |
tet (A) | GCTGTTTGTTCTGCCGGAAA | GGTTAAGTTCCTTGAACGCAAACT |
tet (D) | TGCCGCGTTTGATTACACA | CACCAGTGATCCCGGAGATAA |
tet (E) | TTGGCGCTGTATGCAATGAT | CGACGACCTATGCGATCTGA |
tet (M) | CATCATAGACACGCCAGGACATAT | CGCCATCTTTTGCAGAAATCA |
intI | GGCATCCAAGCAGCAAG | AAGCAGACTTGACCTGA |
intI2 | CAC GGA TAT GCG ACA AAA AGG T | GTA GCA AAC GAG TGA CGA AAT G |
intI1 | CGAACGAGTGGCGGAGGGTG | TACCCGAGAGCTTGGCACCCA |
IS613 | AGGTTCGGACTCAATGCAACA | TTCAGCACATACCGCCTTGAT |
tnpA-01 | CATCATCGGACGGACAGAATT | GTCGGAGATGTGGGTGTAGAAAGT |
tnpA-02 | GGGCGGGTCGATTGAAA | GTGGGCGGGATCTGCTT |
tnpA-03 | AATTGATGCGGACGGCTTAA | TCACCAAACTGTTTATGGAGTCGTT |
Fig.1 Absolute abundances of T-ARGs and MGEs in intestinal microorganisms of R.bieti. A: T-ARGs of modification mechanism; B: T-ARGs of ribosomal protection mechanism; C: T-ARGs of expump mechanism; D: MGEs. The same lowercase indicates there are no significant difference between female and male (P ≥ 0. 05). F1 to F4 are the 4 female R.bieti individuals and M1 to M4 are the 4 males R.bieti individuals
Fig. 2 Relative abundances of intestinal microorganisms of R.bieti. A: intestinal microorganisms in phylum level; B: intestinal microorganisms ingenus level. * indicates there are significant difference between female and male (P < 0. 05). F1 to F4 are the 4 female R.bieti individuals and M1 to M4 are the 4 males R.bieti individuals
Fig. 3 Enzyme activity and nutrient content in intestine of R.bieti. The different lowercase indicates there are significant difference between female and male (P < 0.05). F1 to F4 are the 4 female R.bieti individuals and M1 to M4 are the 4 males R.bieti individuals
Fig. 4 Network analysis on T-ARGs, MGEs and microorganisms in the intestine of R.bieti. The red line segments indicate significant positive correlation (P < 0.05), while the green line segments indicate significant negative correlation (P < 0.05). The blue nodes represent microbial species, the yellow nodes represent the T-ARGs and the green nodes represent the MGEs. The size of the dot indicates the number of associations with other species, and the larger the number of related factors, the larger the radius of the dots
指标 Indices | 核糖体保护机制的T-ARGs T-ARGs of protecting ribosomes | 外泵机制的T-ARGs T-ARGs of efflux pump | MGEs | 淀粉酶活力 Amylase activity | 脂肪酶活力 Lipase activity | 蛋白酶活力 Protease activity | 纤维素酶活力 Cellulose activity | 蛋白质含量 Protein content | 氨基酸 含量 amino acids contents |
---|---|---|---|---|---|---|---|---|---|
基因修饰机制的T-ARGs T-ARGs of gene modification | -0.180 | 0.151 | 0.212 | 0.111 | 0.327 | 0.482 | 0.379 | 0.064 | 0.162 |
核糖体保护机制的T-ARGs T-ARGs of protecting ribosomes | 0.336 | -0.149 | -0.079 | -0.215 | -0.101 | -0.092 | -0.452 | -0.574 | |
外泵机制的T-ARGs T-ARGs of efflux pump | -0.439 | 0.461 | -0.445 | -0.323 | -0.378 | -0.641* | -0.710* | ||
MGEs | 0.210 | 0.895** | 0.917** | 0.976** | 0.637* | 0.646* | |||
淀粉酶活力 Amylase activity | 0.395 | 0.201 | 0.147 | -0.374 | -0.251 | ||||
脂肪酶活力 Lipase activity | 0.899** | 0.871** | 0.474 | 0.612 | |||||
蛋白酶活力 Protease activity | 0.966** | 0.512 | 0.577 | ||||||
纤维素酶活力 Cellulose activity | 0.600 | 0.608 | |||||||
蛋白质含量 Protein content | 0.953** |
Table 2 Correlation coefficients between T-ARGs and MGEs in intestinal microorganisms and environmental characteristics in R.bieti
指标 Indices | 核糖体保护机制的T-ARGs T-ARGs of protecting ribosomes | 外泵机制的T-ARGs T-ARGs of efflux pump | MGEs | 淀粉酶活力 Amylase activity | 脂肪酶活力 Lipase activity | 蛋白酶活力 Protease activity | 纤维素酶活力 Cellulose activity | 蛋白质含量 Protein content | 氨基酸 含量 amino acids contents |
---|---|---|---|---|---|---|---|---|---|
基因修饰机制的T-ARGs T-ARGs of gene modification | -0.180 | 0.151 | 0.212 | 0.111 | 0.327 | 0.482 | 0.379 | 0.064 | 0.162 |
核糖体保护机制的T-ARGs T-ARGs of protecting ribosomes | 0.336 | -0.149 | -0.079 | -0.215 | -0.101 | -0.092 | -0.452 | -0.574 | |
外泵机制的T-ARGs T-ARGs of efflux pump | -0.439 | 0.461 | -0.445 | -0.323 | -0.378 | -0.641* | -0.710* | ||
MGEs | 0.210 | 0.895** | 0.917** | 0.976** | 0.637* | 0.646* | |||
淀粉酶活力 Amylase activity | 0.395 | 0.201 | 0.147 | -0.374 | -0.251 | ||||
脂肪酶活力 Lipase activity | 0.899** | 0.871** | 0.474 | 0.612 | |||||
蛋白酶活力 Protease activity | 0.966** | 0.512 | 0.577 | ||||||
纤维素酶活力 Cellulose activity | 0.600 | 0.608 | |||||||
蛋白质含量 Protein content | 0.953** |
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