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Table of Content

    07 July 2008, Volume 24 Issue 1
    Study on Geographical Division of Alpine Musk Deer ( Moschus sifanicus)
    XIA Lin YANGQisen WEI Fuwen LI Ming
    2004, 24(1):  1-5. 
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    Alpine musk deer ( Moschus sifanicusg) is an important economic animal in China. Because of being over-hunting and environmental deterioration , populations of alpine musk deer have become isolated in their distributional area. In order to protect biodiversity , this study inquired about intra-specific genetic diversity among three populations of alpine musk deer (Sichuan , Ganshu , and Xizang) by molecular biological method. 352 bp of mitochondrial cytochrome b gene were sequenced by PCR , using a pair of universal primer (L14724 and H15149) , phylogenetic trees were contracted using maximum parsimony method and neighbor-joining method. The results suggested that these three populations of alpine musk deer may be different , sequence divergenece of cytb was 0.57 % - 2.84 % among the three populations , which was within level of intraspecies. The phylogenetic analysis revealed that they shared a common ancestor. Mega-years ago and the Sichuan population first diverged from the common ancestor. The results also revealed that the Gansu population was close to the Xizang populations ; the Gansu population diverged from the Sichuan population 0.8 Mega-years ago , and the Xizang populations diverged from Gansu population and Sichuan population 0.34 - 0.57 and 0.9 - 1 Mega-years ago. Results implied that these populations in Gansu , Sichuan and Xizang may be different subspecies.
    Habitat Selection and its Seasonal Change of Serow (Capricornis sumatraensis) in Cibagou Nature Reserve , Tibet
    WU Pengju ZHANG Endi
    2004, 24(1):  6-12. 
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    A study on the habitat selection and its seasonal change of serow was conducted in Cibagou Nature Reserve of Zayu ,Tibet from December 2001 to August 2002. 14 line transects perpendicular to contours were set out along the valley , From bottom to upper position , sampling plots (10 m ×10 m) were set every 250 m apart , and 187 plots were made in total. The vegetation types , elevations , slope gradients , canopy covers , shelters and distances fromwater of these plots were recorded. These plots were examined repeatedly to record whether plots were used by the serow recently in winter , spring and summer respectively. Ivlev's Selectivity Index was used to evaluate the selectivity of serow to vegetation and elevations. The frequency of utilization was used to analyze other factors. It was found that serow mainly selected coniferous forest in the winter , and mostly selected Theropencedrymion forests and secondly Fargesia-coniferous forests in the spring. In the summer , serows selected forests with extended ranges , which included theropencedrymion , Fargesia-coniferous forests , and Rhododendron-coniferous forests. In Cibagou Nature Reserve , serow's selection for vegetations correlated significantly with that for elevations ( P < 0.01). We have also found that vegetation and elevation have approximate influence on the distribution of serow. These change of serow's electivity in vegetation types and elevations in different seasons were mainly attributed to the seasonal change of food resource. In the winter and spring , serows mostly utilized flat and slow slopes , while they used steeper slopes in the summer , which was significantly different from winter and spring statistically , and the difference of utilization of slope position in different seasons could account for these change. Serow's utilization for shelter , concealment condition and distance from water did not been influenced significantly by the seasonal change. Serows mostly utilized the places with medium shelter , highest concealment condition , or near water source ,which probably mainly relate to body size and isolated life of serow and distribution of these ecological factors.
    Expression of Biologically Active Neurotrophin -4 of Giant Panda in Escherichia coli
    RAO Gang FANG Shengguo Tsutomu Fujihara WEI Fuwen
    2004, 24(1):  13-18. 
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    The neurotrophin - 4 (NT- 4) gene plays an important role in the protection of the damages resulted from epilepsy ,which is an important cause of death in the pandas. The genomic DNA coding for the NT- 4 gene of the giant panda was cloned into expression vector pGEX- 4T-3 under the control of a tac promoter , and expressed in Escherichia coli . After purification ,the recombinant NT- 4 of the panda was found to be biologically active in the PC12 neurite outgrowth assay. We suggest that this technique may offer a feasible means of gene therapy for the giant pandas suffering from epilepsy.
    Birth of Tibetan Monkey ( Macaca thibetana) in Winter
    YIN Huabao LI Jinhua ZHOU Lizhi GE Jizhi Consuel Ionica Carol M. Berman
    2004, 24(1):  19-22. 
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    On the basis of theory of natural selection , an animal concentrates its breeding time on the period of good condition. In general , seasonal breeding pattern of any primate species is normal. Rare Tibetan macaques (Macaca thibetana) in Mt. Huangshan exhibit seasonal breeding pattern that apparently is maintained intrinsically. Its date of birth normally occur during the months of January and May. However , in 2001 , a case of birth in winter was observed. Although it is an incidental case , studying of these internal and external factors happen to it is of great significance. Based on the sequential birth data of Tibetan macaques and the sequential changing tendency of annual mean air temperature , precipitation , sunlight during 14 years from 1988 to 2001 , using t-test ( r= 0.60) , the results show that there is significance correlation ( P < 0.05) between the birth dates and annual mean air temperature , and no significance between the birth dates and annual mean precipitation and sunlight ( P > 0.05). The paper also analyze the others possible long-term impact of variation of altitude and latitude and translocation and subsequent provisioning and range restriction on the birth time of this species. Available integrate evidence , including rising of annual mean air temperature and translocation and provisioning and range restriction , concerning the variation and prolonging of birth time in this species is suggestive.
    Hay-pile Caches as Winter Food by Gansu Pikas and Its Biological Signif icance
    SU Jianping LIAN Xinming ZHANG Tongzuo CUI Qinghu LIU Jike
    2004, 24(1):  23-29. 
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    Composition of haypile caches as winter food by Gansu pikas ( Ochotona cansus) and it's relationships to habitat vegetation type were studied based on the 27 hay-piles collected in late fall of 1999 from meadow , shrub , and old fields at the Haibei Research Station of Alpine Meadow Ecosystem , the Chinese Academy of Sciences. The results showed that each hay-pile had a dried weight of 23.8 - 984.2 g with average of 184.2 g , and no significant difference was detected between samples collected from meadow , shrub , and old fields. There were 26 species of dicotyledons and some unidentified monocotyledons in Gramineae in the hay-pile materials. The proportion of dicotyledons in dried weight was 84.61 % , much higher than that of monocotyledons (8.06 %). Compared to their proportions in the environment (39.57 % for dicotyledons and 51.76 % for monocotyledons) , this meant that Gansu pikas preferred dicotyledons to monocotyledons when they cached their winter food. Preferences of plant materials were relatively stable , even though their availability in habitats affected their proportions in hay-piles. The most preferring plants were Saussurea superba , Oxytropis spp. , Gentiana straminea , some grasses in Gramineae , and Gentiana farreri. Overwinter survival of Gansu pikas reduced from 51.84 % for control plot ( 1 hm2) to 21133 %for treatment plot ( 1 hm2) by removing hay-piles , implying that cached food was critical for the animal to live over the winter. Field observations indicated that vocal behavior of Gansu pikas developed poor than that of sympatric plateau pika ( Ochotona curzoniae) without the habit of caching winter food. This disagreed with Kawamichi's (1976 , 1985) opinion that the development of calling parallels the occurrence of harvesting behavior.
    Karyology of Seven Species Bats from Sichuan , China
    WU Yi Masashi HARADA LI Yanhong
    2004, 24(1):  30-35. 
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    The karyotypes of seven species bats from Sichuan , China were examined (namely : Rhinolophus cornutus , Rhinolophus sinicus , Hipposideros armiger , Ia io , Pipistrellus abramus , Nyctalus velutinus , and Tadarida teniotis) . It is essentially identical to those previour reports about the karyotypes of Rhinolophus cornutus (2n = 62 , FN = 60) , Hipposideros armiger (2n =32 , FN = 60) , Ia io (2n = 50 , FN = 48) , Pipistrellus abramus (2n = 26 , FN = 44) , Nyctalus velutinus (2n = 36 , FN
    = 54) , and Tadarida teniotis (2n = 48 , FN = 62) . R. sinicus should be named as a species instead of a subspecies of R. rouxii . The karyotype of Ia io is reported for the first time.
    Applicability on Estimating Population Parameters of Mongolian Gerbil (Meriones unguiculatus) by the Jolly-Seber Model
    LIU Wei WAN Xinrong WANG Guanghe LIU Wendong ZHONGWenqin
    2004, 24(1):  36-41. 
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    The population parameters of Mongolian gerbil (Meriones unguiculatus) in Inner Mongolia of China were estimated by using Capture-mark-recapture (CMR) and Jolly - Seber model from June to October in 2000. We found that the possibility of being caught among the individuals in marked population of Mongolian gerbil is not different , that is equal catch-ability ; and the mean probability that an animal kept alive at ith sampling occasion is re-caught at sample i was 89.7 % in each capture-recapture time.It shows that the Jolly-Seber model is suitable for estimating parameters of M. unguiculatus.
    Influence of Locally Experimental Warming on Patches
    SUN Ping ZHAO Xinquan WEI Wanhong XU Shixiao ZHAO Wei ZHAO Tongbiao
    2004, 24(1):  42-47. 
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    To discuss the influence of patch effect of locally experimental warming (EW) on root voles'habitat choice , using topopen chambers (TOC) to simulate global warming , the root vole's captured frequencies in 4 treatments in different grazing-intensityplots were investigated by mark-recapture method. The four treatments included EW, cut grass similating grazing (CUT) , EW &CUT (EWC) , and control (CK). The results showed that : (1) There was no influence of different grazing intensities on root vole's captured frequencies ( P > 0.05) , but among 4 treatments in High Grazing Meadow (HGM) , the differences were significantduring both warm or cold seasons ( P < 0.05) . In Low Grazing Meadow (LGM) , it was not significant in warm season ( P >0.05) , however , it was evident during cold one ( P < 0.05) . (2) There were no significant influence of the ulterior effect ofwarming chambers foundation on habitat selection of root vole , cut grass treatment on habitat choice of root vole had also no effect.In warm season , Locally experimental warming (LEW) had no effect on root vole's habitat choice ( P > 0.05) ; In cold season ,there were significant difference of EW and CK ( P < 0.05) , but EWC and CUT had no difference. The results indicated thatLEW had no influence on root vole's habitat choice in warm season. In cold season , this effect was significant between EW andCK, but it was not significant between EWC and CUT.
    Population and Conservation of Giant Pandas In Wolong and Caopo Nature Reserves
    HU Jinchu
    2004, 24(1):  48-52. 
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    Since 1974 , many investigations on the population and conservation of giant pandas have been carried out in Wolong and Caopo Nature Reserves. The two reserves are located in the eastern foot of Qionglai Mountains , which is the central zone of the five mountains where Chinese giant pandas live.The area is 3 224 km2 , constituting 75.73 %of the total area of the six reserves in Qionglai Mountains and 21.41 % of 35 reserves in China. Located in the high mountain and deep valley belt which transits from Sichuan Basin to Qinghai-Tibet Plateau, there are advantageous natural conditions in the two reserves. Their faunas are old and complex , and their biodiversity also is extremely abundant. According to the relevant statistics , there are over 400 species of higher plants in the two reserves and 481 terrestrial vertebrate , species that constitutes 45.38 % in Sichuan. Besides the giant panda , these two reserves are also the distributive central belt for Rhinopithecus roxellana , Budorcas taxicolor , and so on , which are rare and endangered animals. Furthermore , there are 100 Chinese native terrestrial vertebrate in the two reserves constituting 37% of that in Sichuan. As to the habitat selection of the giant pandas , the most desirable habitats lie in the third or fourth plane with 3×104 hm2 at an altitude of 2 800 - 3 100 m , where there are gentle landforms , prosperous vegetations , green bamboos and abundant water sources. The more desirable habitats selected by part of the giant pandas in the summer with 2×104 hm2 are located at an altitude of 3 100 - 3 600 m , where slopes are steeper and vegetations are lower with cold winter and cool summer.The habitats with worse quality are located at an altitude of 2 100 - 2 500 m , where the vegetation is needle-and-broad-leaf-mixed forests and has been relatively destroyed severely. As leaves on many trees fall, sunshine is reinforced and there is little snow on the ground. Some panda individuals may utilize this sort of habitats in the winter , or they eat bamboo shoot there in the summer.It covers 8 000 hm2.The last sort of habitats with the worst quality are below the altitude of 2 100 m , down to valleys , where the gentle slopes have been turned into croplands and the forests only exist in steep valleys. A few pandas go down there to have bamboo leaves and drink and the area of that is 12 000 hm2 or so. According to the investigation in 1974 , there are 195 giant pandas in those two reserves with 145 of them.Sine Bashania fangiana bloomed and died off widely in 1983 , the number of panda population decreased and only about 100 pandas survived , which was based on incomplete survey after the calamity. However , it should be mentioned that several graduate students , who studied at the Nanchong Teachers College made similar survey and their results indicated more than 100 pandas survived in the two reserves. In 1998 , another investigation was carried out , indicating over 150 pandas living there. That indicated the number of the panda population had been increased gradually. According to the life table , which is based on the age determination of incisor sections of the skulls collected in the field , the net reproduction rate (R0) is 1.067 2 and the mean generation time is 11.679 year. The viability of Wuyipeng giant panda population has also been analyzed.Its results also show the net reproduction rate R0 > 1. There are significant differences for the genetic diversity of giant panda among the five mountains , and Qionglai Mountains takes the first place. Based all above researches , the local panda population may gradually increase at present. As to conservative strategies , croplands should be returned back to original forests to make it possible for the separated local population to diffuse and migrate one another. The rate of traffic flow should also be limited on the road , which crosses transversely Wolong Nature Reserve , especially prohibiting car driving at night. The absolute sealed of the conservation should be in place to protect in the most desirable habitats for giant pandas , and the area for traveling and the total number of travelers should be effectively controlled to reduce human disturbance to the minimum. It may be more appropriate to extend these two reserves to the edge area where a few giant pandas live , for a larger reproductive population may exists much longer. The target of above strategies , which may be adapted , is to reduce the extinction rate , to add gene heterozygosity and to make it possible for giant panda population to exist permanently.
    Review on Dominance Hierarchy of Non-human Primates
    LI Hongqun ZHANG Yuhui LI Baoguo
    2004, 24(1):  53-60. 
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    Dominance in some animals has been reported , especially in non-human primates. Dominance in non-human primates and its relationships with others factors were discussed in this review. The conclusion of this paper revealed that dominance is determined by individual factors such as age , sex , proximity , maternal rank and tenure , but also is displayed by some social interactions including allogroom , aggression , mount and so on. Dominance is various in different species , so it is necessary to adopt different index to evaluate dominance in different animals. In general , dominance benefited to the whole groups and individuals to some extent.
    Hoarding Behavior of Rodents and Plant Seed Dispersal
    XIAO Zhishu ZHANG Zhibin
    2004, 24(1):  61-70. 
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    This paper reviews research achievements on rodents'hoarding behavior of plant seeds and fruits , and aims to provide resources for future studies on their roles in the dispersal of plant seeds and fruits. Most rodents consume large quantities of plant seeds and fruits. On the other hand , they disperse plant seeds and fruits away from parent trees or other seed sources via their hoarding behavior and scattering them under the litters or in the shallow layers of the surface soil. This may influence spatio-temporal distribution of the seeds and fruits , and consequently provide advantageous conditions for seed germination and seedling establishment. It has widely evolved a mutually beneficial or co-evolutionary relationship between rodents and plant seeds or fruits. Rodents' hoarding behavior may affect the dispersal of plant seeds and fruits via the following main processes : selection , transportation , burial (ie. scatter-hoarding) of the plant seeds and fruits ; and their consequential impacts on the survival and mortality of seeds and seedlings.
    Inter-Simple Sequence Repeat and Application
    XIE Jiayan ZHANG Zhibin
    2004, 24(1):  71-77. 
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    The purpose of this review is to introduce principles , methods , characteristics and applications of a new DNA marker ,inter-simple sequence repeat ( ISSR) , which involves the use of microsatellite sequences directly in the polymerase chain reaction (PCR) for DNA amplification. ISSR using SSR - based primers is a very useful systemfor efficient retrieval and analysis of genetic information in eukaryotes. The ISSR technique tests fast , requires very little genomic sequence data and screens so many polymorphisms in an assay. The advantages of ISSR should make this marker system used widely in the research of genetic diversity.
    Activity Synchrony and Aggregation Tendency in Père David's
    ZENG Yan JIANG Zhigang LI Chunwang YAN Caie ZHANGLinyuan XIA Jingshi TANG Baotian
    2004, 24(1):  78-81. 
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    In 1999 , we observed six Père David's deer calves (3 male and 3 female) , which were born in the Beijing Milu Park to study the synchronization of action and grouping behavior in those calves. The observation began in the second week of their births until their weaning , altogether , we collected 4 320 behavioral records. We established two indexes , the Index of Activity Synchrony , A , and Daily Frequency of Calves in the Same Cohort , P , for measuring aggregate behaviour in calves. Before their weaning , the calves synchronised their activity (A = 0.87 ±0.006) , and they stayed in same cohort most of the time ( P = 0.86±0.012). No sexual segregation behaviour was found in the calves. There were no significant differences of A ( F = 0.075 , P= 0.788 , df = 1) and P ( F = 0.489 , P = 0.497 , df = 1) in the calves of the same sex or between opposite sex. However , Index A and P changed significantly as the ages of the calves increased. Daily frequency of calves stayed in the same cohort could not present aggregation tendency of calves exactly. At 4 week old , calves expressed the highest activity synchronization.
    Preliminary Observation on Eco-biology of Captive Red
    GUO Wenli PEI Enle LI Zhongkui WANGAishan TU Rongxiu
    2004, 24(1):  82-83. 
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    This study presents the results of a demographic analysis of 20 years of birth and death records of red gorals kept at the Shanghai Zoo. The age of the first successful breeding , sex ratio at birth , seasonality of reproduction and mortality rate are presented. The females or males of red goral born in captivity can give their first birth at the age of 2 or 3 separately. The births mainly occurred from May to July and peaked in June. Ratio of females to males at birth was 2.36∶11 The mortality rate for infants and adults were 23.1 % and 11.8 % , respectively while there was no sub-adult death recorded during the 20 years period. There was a definite relation between mortality rate and seasons and mainly in July.
    Survey on Population of the Golden Monkey ( Rhinopithecus
    SU Hualong MA Qiang LIN Yinghua CHEN Qianming QIAO Yungao TAN Mingfeng
    2004, 24(1):  84-87. 
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    The population activities of golden monkeys ( Rhinopithecus roxellana) in the Xiaoshennongjia of Badong county , Hubei Province had been surveyed by consecutively tracing and grossly counting from Oct. 2000 to June 2002. The result indicated the amount of the golden monkey that inhabit in here about 600 - 800 individuals. They moved clockwise in a circular track. 236 golden monkeys of 2 groups had recorded by video camera during survey in Apr. 2001 , among them there were 59.05 % adult and 40.95 % of underage , the rate was 1∶0.9 , the sex ratio of adult was 1∶1.38 (male : female) . 117 individuals of three different groups were recorded in random in 2002 , among themwere 56.41 %of adult and 43.59 % underage , the rate was 1∶0.67 , the sex ratio of adult was 1∶1.21 The young were born from the beginning the month of March.
    Primary Observations on Portal Venous System Within Liver of Neophocaena phocaenoides asiaeorientalis
    CHEN Jiaji
    2004, 24(1):  88-89. 
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    The portal venous system within liver of Neophocaena phocaenoides asiaeorientalis was observed. The portal venous system is similar to other mammals (pig , rabbit , ox , dog , goat , rhesus monkey). The R. sinister of the portal vein gives off the v.dorsalis lobi sin lat. , the v. ventralis lobi sin.lat. , the v. lateralis lobi sin.med. , the v. medialis lobi sin , med. , Rr.lobi caudati and Rr.lobi quadrati ; the R. dexter of portal vein gives off the v. lobi dext1med. , the v. lobi dext.lat. and the V. processus caudati. It seems that the portal venous system of aquatic mammals and terrestrial mammals show no obvious difference.
    ISSR Fingerprint for Manchurian Tigers in Captivity
    BAI Xiujuan
    2004, 24(1):  90-92. 
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    Manchurian tigers kept in Hengdaohezi Breeding Center , Heilongjiang province , were fingerprinted using inter simple sequence repeated ( ISSR) technique. Seven primers were screened out of 20 primers with 41 sites deprived , The results indicated that 31 bands were polymorphic sites and the frequency was 76%. The maximum genetic similarity coefficients among the 15 tigers were 0.526 3 and 0.090 9 , respectively. The average genetic similarity coefficient of the population was 0.327 1.