冬季不同海拔大绒鼠MC1R基因的表达量及UCP1、Hb、Mb含量的比较

doi:10.16829/j.slxb.150562

兽类学报 ›› 2022, Vol. 42 ›› Issue (1): 69-75.DOI: 10.16829/j.slxb.150562

冬季不同海拔大绒鼠MC1R基因的表达量及UCP1、Hb、Mb含量的比较

韩春艳¹, 贾婷², 王炎¹, 王政昆¹, 朱万龙¹, 张浩¹

1 云南省高校西南山地生态系统动植物生态适应进化及保护重点实验室, 云南师范大学生命科学学院, 昆明 650500;
2 云南经济管理学院, 昆明 650106

收稿日期:2021-05-07 修回日期:2021-10-18 出版日期:2022-01-30 发布日期:2022-01-13
通讯作者: 朱万龙,E-mail:zwl_8307@163.com;张浩,E-mail:aple20@sina.com
作者简介:韩春艳(1996-),女,硕士研究生,主要从事动物生理生态的研究.E-mail:hanchunyaner@163.com
基金资助:
国家自然科学基金（31760118，32160254）；云南省中青年学术和技术带头人后备人才项目（2019HB013）；云南省万人计划青年拔尖人才项目（YNWR-QNRC-2019-047）

Comparative on the expression of MC1R gene and the contents of UCP1, Hb and Mb of Eothenomys miletus at different altitudes from Hengduan Mountain region in winter

HAN Chunyan¹, JIA Ting², WANG Yan¹, WANG Zhengkun¹, ZHU Wanlong¹, ZHANG Hao¹

1 Key Laboratory of Ecological Adaptive Evolution and Conservation on Animals-Plants in Southwest Mountain Ecosystem of Yunnan, School of Life Sciences, Yunnan Normal University, Kunming 650500, China;
2 Yunnan College of Business Management, Kunming 650106, China

Received:2021-05-07 Revised:2021-10-18 Online:2022-01-30 Published:2022-01-13

摘要/Abstract

摘要： 为探究横断山冬季不同海拔大绒鼠的毛色差异、生理及血液指标的变化情况，自北向南从德钦、香格里拉、丽江、剑川和哀牢山5个地区按高海拔至低海拔进行采样，通过PCR扩增大绒鼠黑素皮质素受体1（melano-cortin receptor 1，MC1R）基因序列，使用FQ-PCR测定其皮肤的MC1R基因表达量，并利用酶联免疫分析法（ELI-SA）测定褐色脂肪组织中解偶联蛋白1（uncoupling protein 1，UCP1）含量、血清中血红蛋白和肌红蛋白含量。结果表明：扩增出的MC1R基因片段为约200 bp的部分序列；哀牢山和剑川地区的大绒鼠MC1R基因表达量显著高于丽江、香格里拉和德钦地区；德钦和香格里拉地区的UCP1含量和肌红蛋白含量显著高于哀牢山、剑川和丽江地区；5个地区间血红蛋白含量差异不显著。以上结果表明大绒鼠通过改变MC1R基因的表达量来改变毛色，还通过升高肌红蛋白含量和UCP1含量来增加产热能力，从而适应横断山不同海拔的环境变化。

关键词: 大绒鼠, 毛色基因, UCP1, 血红蛋白, 肌红蛋白

Abstract: In order to explore the variations of coat color, physiological and blood indexes in Eothenomys miletus at different elevations in winter, samples were collected from north to south of Hengduan Mountain region in Deqin (DQ), Shangri-la (XGLL), Lijiang (LJ), Jianchuan (JC) and Ailao Mountain (ALS) from north to south, Gene sequence of melanocortin receptor1 (MC1R) was amplified by PCR and the expression of MC1R gene in skin was determined by FQPCR. The contents of uncoupling protein 1 (UCP1) in brown adipose tissue, hemoglobin (Hb) and myoglobin (Mb) in serum were measured by enzyme-linked immunoassay (ELISA). The results showed that the amplified MC1R fragment was a partial sequence of about 200 bp. MC1R gene expression in E. miletus from ALS and JC was significantly higher than from LJ, XGLL and DQ. Contents of UCP1 and Mb in E. miletus from DQ and XGLL were significantly higher than that those from ALS, JC and LJ. But Hb content showed no significant difference among the five regions. All of the above results showed that E. miletus could change the coat color by changing the expression of MC1R gene and increase the thermogenesis capacity by elevating the content of Mb and UCP1 in order to adapt to the environmental changes at different altitudes from Hengduan Mountain region.

Key words: Eothenomys miletus, Coat color gene, UCP1, Hemoglobin, Myoglobin

中图分类号:

Q494

韩春艳, 贾婷, 王炎, 王政昆, 朱万龙, 张浩. 冬季不同海拔大绒鼠MC1R基因的表达量及UCP1、Hb、Mb含量的比较[J]. 兽类学报, 2022, 42(1): 69-75.

HAN Chunyan, JIA Ting, WANG Yan, WANG Zhengkun, ZHU Wanlong, ZHANG Hao. Comparative on the expression of MC1R gene and the contents of UCP1, Hb and Mb of Eothenomys miletus at different altitudes from Hengduan Mountain region in winter[J]. ACTA THERIOLOGICA SINICA, 2022, 42(1): 69-75.

参考文献

Aquila H, Link T A, Klingenberg M. 1985. The uncoupling protein from brown fat mitochondria is related to the mitochondrial ADP/ATP carrier. Analysis of sequence homologies and of folding of the protein inthe membrane. The EMBO Journal, 4 (9):2369-2376.
Astrand I, Astrand P, Christensen E, Hedman R. 2010. Myohemoglobinasan oxygen-store in man. Acta Physiologica Scandinavica, 48 (3-4):454-460.
Ayer G, Grandchamp A, Wyler T, Trunigeret B. 1971. Intrarenal hemodynamics in glycerol-induced myohemoglobinuric acute renal failure in the rat. Circulation Research, 29 (2):128-135.
Bouillaud F, Ricquier D, Thibault J, Weissenbach J. 1985. Molecular approach to thermogenesis in brown adipose tissue:cDNA cloning of the mitochondrial uncoupling protein. Proceedings of the National Academy of Sciences, 82 (2):445-448.
Christoforides C, Hedley-Whyte J. 1969. Effect of temperature and hemoglobin concentration on solubility of O₂ in blood. Journal of Applied Physiology, 27 (5):592-596.
Cone R D, Lu D, Koppula S, Vage D I, Klungland H, Boston B, Chen W, Orth D N, Pouton C, Kesterson R A. 1996. The melanocortin receptors:agonists, antagonists, and the hormonal control of pigmentation. Recent Progress in Hormone Research, 51:287-317.
Cunningham S, Leslie P, Hopwood D, Lllingworth P, Jung R T, Nicholls D G, Peden N, Rafael J, Rial E. 1985. The characterization and energetic potential of brown adipose tissue in man. Clinical Science (London, England:1979), 69 (3):343-348.
Feng J Q, Xu W, Huang L, Luo W X, Cai H F. 2017. Research progress of MC1R gene. China Animal Husbandry & Veterinary Medicine, 44 (4):1141-1148. (in Chinese)
Fontanesi L, Dall' Olio S, Beretti F, Portolano B, Russo V. 2011. Coat colours in the Massese sheep breed are associated with mutations in the agouti signaling protein (ASIP) and melanocortin 1 receptor(MC1R) genes. Animal, 5 (1):8-17.
Golozoubova V, Hohtola E, Matthias A, Jacobsson A, Nedergaard J. 2001. Only UCP1 can mediate adaptive nonshivering thermogenesis in the cold. Faseb Journal, 15 (11):2048-2050.
Gong Z D, Wu H Y, Duan X D, Feng X G, Zhang Y Z, Liu Q. 2001. Species diversity and geographical distribution of small mammals in Hengduan Mountain. Biodiversity Science, 9 (1):73-79. (in Chinese)
Guan Z H, Ma C Y, Fei H L, Huang B, Ning W H, Ni Q Y, Jiang X L, Fan P F. 2018. Ecology and social system of northern gibbons living in cold seasonal forests. Zoological Research, 39 (4):255-265.
Han C Y, Jia T, Yang J, Wang Z K, Zhu W L. 2021. Body mass regulation by Eothenomys miletus at different areas of the Hengduan Mountains in winter. Chinese Journal of Wildlife, 42 (1):86-91.(in Chinese)
Hou D M, Jia T, Tao J Q, Wang Z K, Guan B R, Zhu W L. 2021. Browning plasticity of white adipose tissue in tree shrew during cold acclimation and rewarming. Journal of Vertebrate Biology, 70 (1):20097.
Jay F S. 2007. Hemoglobin function and physiological adaptation to hypoxia in high-altitude mammals. Journal of Mammalogy, 88(1):24-31.
Jia X B, Ding P, Chen S Y, Zhao S K, Wang J, Lai S J. 2021. Analysis of MC1R, MITF, TYR, TYRP1, and MLPH genes polymorphism in four rabbit breeds with different coat colors. Animals, 11 (1):81.
Jin Y T, Tong H J, Shao G, Li J S, Lv Y D, Wo Y B, Brown R P, Fu C Y. 2020. Dorsal pigmentation and its association with functional variation in MC1R in a lizard from different elevations on the Qinghai-Tibetan Plateau. Genome Biology and Evolution, 12 (12):2303-2313.
Klungland H, Vage D. 2000. Molecular genetics of pigmentation in domestic animals. Current Genomics, 1 (3):223-242.
Lei J M, Zhu Z Y, Huang Y X. 2012. Changes of hemoglobin size in crocodile blood with concentration, temperature and pH. Chinese Journal of Medical Physics, 29 (3):3420-3422. (in Chinese)
Li X, Li W B, Feng S L, Wang R. 2019. Research progress on mechanism in adaptation of hemoglobin to plateau hypoxia. Joural of Zhejiang University (Medical Sciences), 48 (6):674-681. (in Chinese)
Liu X H, Liang H C, Huang L L. 1981. Physical properties of soil moisture in the northern part of Hengduan Mountains. Journal of Yunnan University (Natural Science), 43 (2):46-64. (in Chinese)
Luo Z X, Chen W, Gao W. 2000. Fauna of China. Beijing:Science Press, 89-95. (in Chinese)
Ma L, Shao X, Wang Y, Yang Y, Bai Z, Zhao Y, Jin G, Ga Q, Yang Q, Ge R L. 2014. Molecular cloning, characterization and expression of myoglobin in Tibetan antelope (Pantholops hodgsonii), a species with hypoxic tolerance. Gene, 533 (2):532-537.
Mac E H, Victor J H, Jiang J W. 1996. Melanocortin receptors:identification and characterization by melanotropic peptide agonists and antagonists. Pigment Cell & Melanoma Research, 9 (5):213-234.
Marketa Z, Klaus H, Good J M, José M, Célio A P, Scott M L. 2018. Function and underlying mechanisms of seasonal color moulting in mammals and birds:what keeps them changing in a warming world? Biological Reviews, 93 (3):1478-1498.
Nowacka-Woszuk J, Salamon S, Gorna A, Switonski M. 2014. Missense polymorphisms in the MC1R gene of the dog, red fox, arctic fox and Chinese raccoon dog. Journal of Animal Breeding & Genetics, 130 (2):136-141.
Nummela A, EronenT, Koponen A, Tikkanen H, Peltonen J E. 2020. Variability in hemoglobin mass response to altitude training camps. Scandinavian Journal of Medicine & Science in Sports, 31 (1):1-8.
Pan Q L, Tang Y H, Xie J, Xie H C, Li J G, He J P. 2015. Comparison of physicochemical characteristics of blood of plateau zokor and SD rats under constant oxygen condition. Chinese Journal of Zoology, 50 (6):837-843. (in Chinese)
Storz J F. 2007. Hemoglobin function and physiological adaptation to hypoxia in high-altitude mammals. Journal of Mammalogy, 88(1):24-31.
Vaibhav A D, Jochen B, Kumar V S. 2021. Methemoglobin formation in mutant hemoglobin α chains:electron transfer parameters and rates. Biophysical Journal, 120 (17):3807-3819.
Veloso C, Bozinovic F. 1993. Dietary and digestive constraints on basal energy metabolism in a small herbivorous rodent. Ecology, 74 (7):2003-2010.
Voltura M B, Wunder B A. 1998. Effects of ambient temperature, diet quality, and food restriction on body composition dynamics of the prairie vole, Microtus ochrogaster. Physiological Zoology, 71 (3):321-328.
Wang F N, Tang M X, Chen Z, Xie H C, Zhang Y. 2015. Determination of blood index of pika at different altitude. Qinghai Journal of Animal Science and Veterinary Medicine, 45 (2):15-16. (in Chinese)
Wang Z K, Gao W R, Zhu W L. 2015. Research progress on metabolic characteristics of three small mammals in Hengduan Mountains. Acta Theriologica Sinica, 35 (4):445-452. (in Chinese)
Wei D B, Wei L. 2001. Determination of erythrocyte hemoglobin and myoglobin in the plateau zokor rat. Journal of Qinghai University (Natural Science Edition), 19 (4):11-12. (in Chinese)
Xue L N, Luo H D, Zhou S H, Wang X, Mao Y Y. 2021. Differential expression of MC1R, Agouti and TYR genes in skin tissues of sheep with different color. Chinese Journal of Animal Science, 57(1):91-95,100. (in Chinese)
Ye F Y, Wang Z K, Zhu W L. 2016. Effects of cold acclimation on the thermogenic activity of brown adipose tissue in Eothenomys miletus. Journal of Yunnan Normal University (Natural Science Edition), 36 (3):61-65. (in Chinese)
Zhang J Z, Dong C S, Fan R, He X Y. 2006. Advances in the formation of fur color in mammals. Advances in Veterinary Medicine, 27 (S1):65-68. (in Chinese)
Zheng S H. 1993. Fossils of Quaternary Rodents from Sichuan and Guizhou. Beijing:Science and Technology Press, 67-82. (in Chinese)
Zhu W L, Yang G. 2017. Role of hypothalamic neuropeptides genes expression on body mass regulation under different photoperiods in Yunnan red-backed vole, Eothenomys miletus. Pakistan Journal of Zoology, 49 (2):647-654.
王政昆, 高文荣, 朱万龙. 2015. 横断山区三种小型哺乳动物代谢特征的研究进展. 兽类学报, 35 (4):445-452.
王福宁, 唐美霞, 陈志, 谢惠春, 张莹. 2015. 不同海拔高原鼠兔的血象指标测定. 青海畜牧兽医杂志, 45 (2):15-16.
叶芳艳, 王政昆, 朱万龙. 2016. 冷驯化对大绒鼠褐色脂肪组织产热活性的影响. 云南师范大学学报 (自然科学版), 36 (3):61-65.
刘醒华, 梁罕超, 黄礼隆. 1981. 横断山系 (北段) 山地土壤的水分物理性质. 云南大学学报 (自然科学版), 43 (2):46-64.
李雪, 李文斌, 封士兰, 王荣. 2019. 血红蛋白在高原低氧适应中的机制研究进展. 浙江大学学报 (医学版), 48 (6):674-681.
张俊珍, 董常生, 范瑞文, 赫晓燕. 2006. 哺乳动物毛色形成研究进展. 动物医学进展, 27 (S1):65-68.
罗泽珣, 陈卫, 高武. 2000. 中国动物志. 北京:科学出版社, 89-95.
郑绍华. 1993. 川黔地区第四纪啮齿动物化石类. 北京:科技出版社, 67-82.
封竣淇, 徐伟, 黄兰, 罗卫星, 蔡惠芬. 2017. MC1R 基因的研究进展. 中国畜牧兽医, 44 (4):1141-1148.
龚正达, 吴厚永, 段兴德, 冯锡光, 张云智, 刘泉. 2001. 云南横断山区小型兽类物种多样性与地理分布趋势. 生物多样性, 9 (1):73-79.
韩春艳, 贾婷, 杨忌, 王政昆, 朱万龙. 2021. 横断山不同地区大绒鼠冬季体重调节. 野生动物学报, 42 (1):86-91.
雷健明, 朱志远, 黄耀熊. 2012. 鳄鱼血血红蛋白大小随浓度、温度和 pH的变化. 中国医学物理学杂志, 29 (3):3420-3422.
潘巧琳, 唐燕红, 谢静, 谢惠春, 李金钢, 何建平. 2015. 常氧条件下高原鼢鼠、甘肃鼢鼠及 SD大鼠血液理化特征比较. 动物学杂志, 50 (6):837-843.
薛丽娜, 罗惠娣, 周胜花, 王曦, 毛杨毅. 2021. 不同毛色绵羊皮肤组织中 MC1R、Agouti 及 TYR 基因差异表达研究. 中国畜牧杂志, 57 (1):91-95,100.
魏登邦, 魏莲. 2001. 高原鼢鼠的红细胞、血红蛋白及肌红蛋白的测定结果. 青海大学学报 (自然科学版), 19 (4):11-12.

[1]	陈辉宝, 贾婷, 章迪, 张浩, 王政昆, 朱万龙. 外源瘦素注射对昆明和大理地区大绒鼠适应性产热的影响[J]. 兽类学报, 2023, 43(1): 21-32.
[2]	陶洁琼贾婷刘璐朱万龙王政昆张浩. 冷驯化及复温对中缅树鼩白色脂肪组织中Ucp1和Cd137表达的影响[J]. 兽类学报, 2020, 40(2): 170-174.
[3]	张海姬侯东敏梅丽谌立新王政昆朱万龙. 夏季横断山不同地区大绒鼠脏器和消化道形态的差异[J]. 兽类学报, 2018, 38(5): 499-503.
[4]	余婷婷周庆宏何丽娟朱万龙张浩高文荣王政昆. 大绒鼠幼仔的生长发育和产热特征[J]. 兽类学报, 2014, 34(2): 158-163.
[5]	章迪周庆宏何丽娟朱万龙王政昆杨盛昌高文荣沐远郑佳. 大绒鼠在不同驯化温度下产热和能量代谢的变化[J]. , 2013, 33(4): 344-351.
[6]	高文荣朱万龙曹能孟丽华王政昆余婷婷沐远郑佳章迪. 禁食和重喂食对大绒鼠体重、产热和血清瘦素的影响[J]. , 2013, 33(2): 106-112.
[7]	黄春梅朱万龙杨盛昌余婷婷王政昆. 大绒鼠NPY、AgRP、POMC 和CART 基因部分序列扩增与分析[J]. , 2013, 33(2): 186-192.
[8]	杨盛昌朱万龙黄春梅余婷婷高文荣王政昆. 食物限制对雄性大绒鼠能量代谢特征的影响[J]. , 2013, 33(1): 55-62.
[9]	朱万龙, 杨永宏, 贾婷, 练硝, 王政昆, 龚正达, 郭宪国. 横断山两种小型哺乳动物的蒸发失水与体温调节[J]. , 2008, 28(1): 65-74.
[10]	王海,杨晓密,刘春燕,王政昆. 大绒鼠和高山姬鼠的体温调节和产热特征[J]. , 2006, 26(2): 144-151.
[11]	杨明　李庆芬黄晨西. 侧脑室注射CRH 对急性冷暴露长爪沙鼠BAT 产热的影响及产热调节机理[J]. , 2003, 23(1): 45-51.
[12]	王政昆,刘璐, 梁子卿,李庆芬,孙儒泳. 大绒鼠体温调节和产热特征[J]. , 1999, 19(4): 276-286.
[13]	黄贵萍　黄钦皂　郭光红廖若男刘　菲. 野生大家鼠和实验大白鼠血红蛋白和乳酸脱氢酶同工酶的比较研究[J]. , 1997, 17(3): 216-220.
[14]	金晓玲，何新霞，周虞灿，吴林富，干常春. 六种鼠苹果酸脱氢酶、血浆蛋白质和血红蛋白的变异分析[J]. , 1996, 16(3): 217-221.
[15]	周虞灿, 景增春, 温得启, 胡晓梅. 几种害鼠血红蛋白型的研究[J]. , 1985, 5(1): 17-23.

冬季不同海拔大绒鼠MC1R基因的表达量及UCP1、Hb、Mb含量的比较

Comparative on the expression of MC1R gene and the contents of UCP1, Hb and Mb of Eothenomys miletus at different altitudes from Hengduan Mountain region in winter

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics