分布于贵州的一种小麝鼩分类地位的厘定

doi:10.16829/j.slxb.150936

摘要/Abstract

摘要： 2018—2023年，在贵州省毕节市七星关区野角乡和六盘水市杨梅乡慕尼克村采集麝鼩属待鉴定标本(Crocidura sp.)25号。基于线粒体细胞色素b基因构建的系统进化树显示待鉴定标本以100%的置信度聚为一个进化支。待鉴定标本在系统进化树上与五指山小麝鼩(广西)(C. wuchihensis GX)表现出最近的系统进化关系，且以90%的置信度分为明显的2个进化支。待鉴定标本与麝鼩属其他物种的遗传距离范围是0.070 2~0.181 7，其中与五指山小麝鼩(广西)的遗传距离最小(0.070 2)，但明显大于灰麝鼩(C.attenuata)与安徽麝鼩(C. anhuiensis)的遗传距离(0.036 5)、华南中麝鼩(C. rapax)与安徽麝鼩的遗传距离(0.037 6)、台湾长尾麝鼩(C. kurodai)与大麝鼩(C. lasiura)的遗传距离(0.041 0)及华南中麝鼩与灰麝鼩的遗传距离(0.049 0)。待鉴定标本与我国南方已知可能分布的其他麝鼩属小型物种[山东小麝鼩C. shantungensis、印支小麝鼩C. indochinensis、东阳江麝鼩C. dongyangjiangensis、五指山小麝鼩(海南) C. wuchihensis HN和五指山小麝鼩(广西) C. wuchihensis GX]存在一定形态差异。待鉴定标本与已报道的C.ilensis phaeopus形态较为一致。鉴于形态学上有明显区分特征，且分子生物学鉴定结果支持待鉴定标本为物种分类阶元，因此，待鉴定标本之前可能是一个隐存种——暗黑小麝鼩(C.phaeopus)。其区分特征：体型较小(头体长48.1~63.6 mm)，尾较长(尾长31.5~46.9 mm)；颅骨较小(颅全长15.68~16.91 mm，颅基长14.78~15.84 mm，腭长6.43~7.34 mm，上齿列长6.21~6.70 mm，下齿列长6.04~6.69 mm)；背部呈深黑褐色，腹部呈黑灰色；前、后足背面均呈黑褐色。

关键词: 麝鼩属, Cyt b基因, 形态学, 暗黑小麝鼩, 隐存种

Abstract: From 2018 to 2023, 25 unidentified specimens of the genus Crocidura were collected from Yejiao Township, Qixingguan District, Bijie City and Munike Village, Yangmei Township, Liupanshui City, Guizhou Province, China. The phylogenetic tree shows the Guizhou specimens clustered in a branch with 100% confidence, based on the mitochondrial Cyt b gene, and were sister to Crocidura wuchihensis GX clade with 90% confidence. The genetic distance between the Guizhou specimens and other species of Crocidura is 0. 070 2 - 0. 181 7. The Guizhou specimens have the smallest genetic distance (0. 070 2) from C. wuchihensis GX. This genetic distance is significantly greater than the genetic distance between C. attenuata and C. anhuiensis (0. 036 5), between C. rapax and C. anhuiensis (0. 037 6), between C. kurodai and C.lasiura (0. 041 0), and between C. rapax and C. attenuata (0. 049 0). There are some morphological differences between the Guizhou specimens and other smaller species of Crocidura, which are known to be possibly distributed in southern China, such as C. shantungensis, C. dongyangjiangensis, C. wuchihensis HN, and C. wuchihensis GX. The morphology of the Guizhou specimens is consistent with that of C. ilensis phaeopus. In view of the obvious distinguishing characteristics in morphology and the molecular results, the Guizhou specimens are treated as a species unit and should be named C. phaeopus. Morphological characteristics of C. phaeopus include small body size (head and body length 48. 1 - 63. 6 mm); long tail (tail length 31. 5 - 46. 9 mm), and small skull (greatest length of skull 15. 68 - 16. 91 mm; condyloincisive length 14. 78 - 15. 84 mm; palatoincisive length 6. 43 - 7. 34 mm; upper toothrow length 6. 21 - 6. 70 mm; lower toothrow length 6. 04 - 6. 69 mm). The dorsum is deep dark brown and the venter is dark gray. The dorsum of the front and back feet is dark brown.

Key words: Crocidura, Cyt b gene, Morphology, Crocidura phaeopus, Cryptic species

中图分类号:

Q959

刘铸, 张璐, 郭秋颖, 张智慧, 蒋纹静, 田新民, 金志民, 张隽晟. 分布于贵州的一种小麝鼩分类地位的厘定[J]. 兽类学报, 2025, 45(4): 537-547.

LIU Zhu, ZHANG Lu, GUO Qiuying, ZHANG Zhihui, JIANG Wenjing, TIAN Xinmin, JIN Zhimin, ZHANG Junsheng. Taxonomic status of a small white-toothed shrew (Crocidura sp.) distributed in Guizhou Province[J]. ACTA THERIOLOGICA SINICA, 2025, 45(4): 537-547.

参考文献

Abramov A V, Meschersky I G, Rozhnov V V. 2009. On the taxonomic status of the Harvest Mouse Micromys minutus (Rodentia:Muridae) from Vietnam [J]. Zootaxa, 2199 (1): 58-68.
Allen G M. 1923. New Chinese insectivores [J]. American Museum Novitates, 100: 1-11.
Bickford D, Lohman D J, Sodhi N S, Ng P K, Meier R, Winker K, Ingram K K, Das I. 2007. Cryptic species as a window on diversity and conservation [J]. Trends in Ecology Evolution, 22 (3):148-155.
Burgin C J, He K. 2018. Family Soricidae (shrews) [A]. In: Wilson D E, Mittermeier R A eds. Handbook of the Mammals of the World. Vol. 8. Insectivores, Sloths and Colugos [M]. Barcelona, Spain:Lynx Editions.
Chen S D, Qing J, Liu Z, Liu Y, Tang M, Murphy R W, Pu Y T, Wang X M, Tang K Y, Guo K J, Jiang X L, Liu S Y. 2020. Multilocus phylogeny and cryptic diversity of white-toothed shrews (Mammalia, Eulipotyphla, Crocidura) in China [J]. BMC Evolutionary Biology, 20: 1-14.
Chen Z, Pei X, Song J, Song W, Shi Z, Onditi K O, Jiang X. 2023. Systematics and evolutionary history of the genus Micromys(Mammalia: Rodentia: Muridae) [J]. Mammalian Biology, 103(4): 389-403.
Dubey S, Zaitsev M, Cosson J F, Abdukadier A, Vogel P. 2006. Pliocene and Pleistocene diversification and multiple refugia in a Eurasian shrew (Crocidura suaveolens group) [J]. Molecular Phylogenetics and Evolution, 38 (3): 635-647.
Heaney L R, Timm R M. 1983. Systematics and distribution of shrews of the genus Crocidura (Mammalia: Insectivora) in Vietnam [J]. Proceedings of the Biological Society of Washington, 96:115-120.
Hoffmann R S. 1987. A review of the systematics and distribution of Chinese red-toothed shrews (Mammalia: Soricinae) [J]. Acta Theriologica Sinica, 7 (2): 100-139.
Irwin D M, Kocher T D, Wilson A C. 1991. Evolution of the Cytochrome b gene of mammals [J]. Molecular Evolution, 32 (2):128-144.
Jiang X L, Hoffmann R S. 2001. A revision of the white-toothed shrews (Crocidura) of southern China [J]. Journal of Mammalogy, 82 (4): 1059-1079.
Lebedev V, Kovalskaya Y, Solovyeva E, Zemlemerova E, Bannikova A, Rusin M, Matrosova V. 2021. Molecular systematics of the Sicista tianschanica species complex: a contribution from historical DNA analysis [J]. PeerJ, 9. DOI: 10. 7717/peerj. 10759．
Li H T, Li Y Y, Motokawa M, Wu Y, Harada M, Li Y C. 2023. The effectiveness of molecular, karyotype and morphological methods in the identification of morphologically conservative sibling species: an integrative taxonomic case of the Crocidura attenuata species complex in China’s mainland [J]. Animals, 13 (4). DOI:10. 3390/ani13040643.
Li H T. 2022. Phylogeny, species delimitation and phylogeography of Crocidura attenuata species complex in China [D]. Ph. D thesis. Qingdao: Shandong university. (in Chinese)
Liu Y, Chen S D, Liu B Q, Liao R, Liu Y X, Liu S Y. A new species of the genus Crocidura (Eulipotyphla: Soricidae) from Zhejiang Pvovince, eastern China [J]. Acta Theriologica Sinica, 40 (1): 1-12. (in Chinese)
Liu Z, Han M F, Gao Y, Zhao J Y, Wang Q Q, Zhang C F, Jin Z M, Tian X M, Zhang J S. 2023. Taxonomic and molecular phylogenetics analyses of Micromys in China [J]. Chinese Journal of Zoology, 58 (6): 853-866. (in Chinese)
Liu Z, Zhang J S, Bai W, Liu H, Xie R X, Yang X, Jin Z M. 2019. Classification and distribution of Soricids in Northeastern China [J]. Acta Theriologica Sinica, 39 (1): 24-42. (in Chinese)
Liu Z, Zhang Z H, Luan T Q, Han M F, Gao Y, Cai H, Chen H, Jin Z M, Zhang J S. 2024. Crocidura dongyangjiangensis found in Liupanshui, Guizhou Province, China [J]. Chinese Journal of Zoology, 59 (1): 115-120. (in Chinese)
Lunde D P, Musser G G, Son N T. 2003. A survey of small mammals from Mt. Tay Con Linh II, Vietnam, with the description of a new species of Chodsigoa (Insectivora: Soricidae) [J]. Mammal Study, 28 (1): 31-46.
Lunde D P, Musser G G, Ziegler T. 2004. Description of a new species of Crocidura (Soricomorpha: Soricidae, Crocidurinae) from Ke Go Nature Reserve, Vietnam [J]. Mammal Study, 29(1): 27-36.
Luo R. 1993. The Mammalian Fauna of Guizhou [M]. Guiyang: Guizhou Science and Technology Press. (in Chinese)
Robinson H C, Kloss C B. 1922. New mammals from French Indo-China and Siam [J]. The Annals and Magazine of Natural History, 9 (49): 87-99.
Shou Z H, Wang S, Lu C K, Zhang L G. 1966. A survey of mammals in Hainan Island [J]. Zoological Systematics, 3 (3): 260-276. (in Chinese)
Smith A T, Xie Y. 2009. A Guide to the Mammals of China [M]. Changsha: Hunan Education Publishing House. (in Chinese)
Stamatakis A. 2014. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies [J]. Bioinformatics, 30(9): 1312-1313.
Tamura K, Stecher G, Kumar S. 2021. MEGA11: molecular evolutionary genetics analysis version 11 [J]. Molecular Biology and Evolution, 38 (7): 3022-3027.
Thompson J D, Gibson T J, Plewniak F, Jeanmougin F, Higgins D G. 1997. The CLUSTAL X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools [J]. Nucleic Acids Research, 25 (24): 4876-4882.
Wei F W, Yang Q S, Wu Y, Jiang X L, Liu S Y. 2022. Taxonomy and Distribution of Mammals in China [M]. Beijing: Science Press.(in Chinese)
Yang Q S, Xia L, Feng Z J, Ma Y, Quan G Q, Wu Y. 2007. A guide to the measurement of mammal skull Ⅴ: Insectivora and Chiroptera [J]. Chinese Journal of Zoology, 42 (2): 56-62. (in Chinese)
Yang Q S, Xia L, Ma Y, Feng Z J, Quan G Q. 2005. A guide to the measurement of mammal skull Ⅰ : Basic measurement [J]. Chinese Journal of Zoology, 40 (3): 50-56. (in Chinese)
Smith A T, 解焱. 2009. 中国兽类野外手册 [M]. 长沙: 湖南教育出版社.
刘洋, 陈顺德, 刘保权, 廖锐, 刘滢珣, 刘少英. 2020. 中国浙江麝鼩属 (劳亚食虫目 : 鼩鼱科) 一新种描记 [J]. 兽类学报, 40(1): 1-12.
刘铸, 张隽晟, 白薇, 刘欢, 解瑞雪, 杨茜, 金志民. 2019. 中国东北地区鼩鼱科动物分类与分布 [J]. 兽类学报, 39 (1): 24-42.
刘铸, 张智慧, 栾天琪, 韩美凤, 高艺, 蔡赫, 陈欢, 金志民, 张隽晟. 2024. 贵州六盘水发现东阳江麝鼩 [J]. 动物学杂志, 59 (1):115-120.
刘铸, 韩美凤, 高艺, 赵婧瑜, 汪青青, 张春凤, 金志民, 田新民, 张隽晟. 2023. 我国巢鼠属分类和分子系统学分析 [J]. 动物学杂志, 58 (6): 853-866.
寿振黄, 汪松, 陆长坤, 张鑾光. 1966. 海南岛的兽类调查 [J]. 动物分类学报, 3 (3): 260-276.
李浩恬. 2022. 中国灰麝鼩物种复合体的系统演化、物种厘定与系统地理 [D]. 青岛: 山东大学博士学位论文.
杨奇森, 夏霖, 马勇, 冯祚建, 全国强. 2005. 兽类头骨测量标准Ⅰ: 基本量度 [J]. 动物学杂志, 40 (3): 50-56．
杨奇森, 夏霖, 冯祚建, 马勇, 全国强, 吴毅. 2007. 兽类头骨测量标准Ⅴ: 食虫目、翼手目 [J]. 动物学杂志, 42 (2): 56-62.
罗蓉. 1993. 贵州兽类志 [M]. 贵阳: 贵州省科技出版社.
魏辅文, 杨奇森, 吴毅, 蒋学龙, 刘少英. 2022. 中国兽类分类与分布[M]. 北京: 科学出版社.

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