Abstract: The red panda (Ailurus fulgens) is an endangered species inhabiting a narrow region between the Himalaya Mountains and the Henduan Mountains． The studbook of captive red pandas in China has 968 registered individuals,of which 355 are living (as of 2013)but in this studbook,some pedigree information is unclear． In order to revise this studbook,microsatellite amplification was conducted on 41 captive red pandas with 19 pairs of red panda specific primers, and paternity relationship was analyzed. The paternity test results and species’original record information were analyzed using Pedigraph Software,and a genetic pedigree of Fuzhou captive red pandas was established．Our findings show that when the mother-child relationship was known,the combined paternity exclusion probability of the 19 loci was 0. 99999968．Genotype reliability testing of the 19 loci in six offspring with known parents revealed that the genotypes of 19 loci of offspring and parents completely comply with Mendelian Law,indicating that these loci can be used to ascertain the paternity of red pandas. The biological fathers of 15 offspring from the Fuzhou captive population were identified successfully. Paternity results showed that every litter has only a single father in spite of mating some female red pandas with multiple males in one breeding season. By genotype comparison,seven pairs of twins in the Fuzhou population were all found to be dizygotic twins and with the exception of studbook individuals #920 and #921,all of the other offspring from the Fuzhou population were sired by two males,#487 and #898,indicating different reproductive success rates．Our findings have practical significance for
strengthening the management of endangered animal populations and designing scientific breeding plans using modern breeding technologies.

Key words: Ailurus fulgens, Genetic pedigree, Microsatellite, Paternity identification

摘要： 小熊猫栖息于喜马拉雅与横断山脉，是亚洲濒危物种。中国的小熊猫谱系已登记圈养小熊猫个体９６８ 只，现有存活个体３５５ 只（２０１３ 年），然而，现有谱系中存在部分信息不明确的问题。为此，我们选择１９ 对小熊猫特异性引物对４１ 只圈养小熊猫进行微卫星扩增，并分析其亲缘关系。将获取的亲子鉴定结果，结合种群原始记录信息，利用Pedigraph 软件构建福州圈养小熊猫的种群遗传谱系图。结果表明：在母子关系确实的情况下，１９个基因座位的联合父权排除概率为０. ９９９９９９６８；利用６ 个已知双亲的后代检验１９ 个基因座位的可信度，后代与双亲的基因型完全符合孟德尔遗传定律，表明１９个基因座位能有效确认小熊猫的亲权关系。应用１９ 个微卫星标记成功地为１５ 个后代找到了生物学父亲。尽管一些雌性个体在繁殖期有多重交配的行为，亲子鉴定的结果显示同一窝小熊猫均来自单一父权。基因型比对结果表明７ 对双胞胎均属于异卵双生子。福州种群的后代中除＃９２０和＃９２１ 外，其余均源于＃４８７ 或＃８９８ 两只雄性，表明不同个体参与繁殖的机会不均等。因此，利用现代繁殖技术，加强濒危野动物种群管理，科学制定繁殖计划具有积极的现实意义。

关键词: 小熊猫, 谱系, 微卫星, 亲子鉴定