The background values of trace elements are used to evaluate the levels in giant pandas, also provide a scientific basis for the prevention, diagnosis of diseases and rational feeding methods. To establish the background values and further improve the database of trace elements in serum of giant panda, this paper presents results from 97 blood samples collected under non-anesthesia condition by caring rearing and medical training of captive giant pandas from CCRCGP. The contents of Fe, Cu, Zn, Mn, Cr, Co, Ni, Pb and Cd in serum determined by atomic absorption spectrometry were 4830 μg/L, 1000 μg/L, 3220 μg/L, 38.37 μg/L, 4.79 μg/L, 23.49 μg/L, 4.76 μg/L, 3.02 μg/L and 1.47 μg/L, respectively. This paper analyzes the micronutrient levels in different sex and age groups by two-factor variance analysis. It is shown that the contents of essential Fe, Cu, Zn, Mn, Cr, Co, Ni were normal, stable, and no significant difference between sex and age. The contents of harmful Pb, Cd were similar in different gender, but there was an accumulation trend with age within the safe range. The trace elements nutritional status in captive giant pandas is comfortable, furthermore, the feeding management and habitat environment suit for giant panda protection.