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Mammalian skeleton specimen preparation using proteolytic enzyme (alcalase)detergent

WU Yonghua,YANG Qisen   

  1. Institute of Zoology,the Chinese Academy of Sciences,Beijing 100101,China
  • Online:2009-06-20 Published:2009-03-08

兽类骨骼标本制作新方法: 碱性蛋白酶消化法

武永华, 杨奇森   

  1. (中国科学院动物研究所,北京100101

Abstract: We report on an easy method to remove muscle tissue from the skeleton by alcalase detergent in mammalian skeleton specimen preparation. In the present paper,using hare skull specimen as an example,we describe the alcalase detergentmethod in details and compare it with other methods for mammalian skeleton specimen preparation. In this method,hare muscle tissues are removed from skeletons by alcalase detergent. With commercial proteinases,alcalase (2.4 L, 2.4 AU/ g, Novozymes Biotechnology Co. , Ltd. ), the process requires around 10 minutes’skeletal simmering in a 0.5% solution (V/V) of the alcalase and water under 60 ℃ condition. In total, about 101 rat skull and 487 hare skull specimens are made using this method. Compared to other traditional methods in mammalian skeleton specimen preparation,the alcalase detergent method is safer,more efficient and less costly,and it is also suitable for batch production of skeleton specimen.

Key words: Alcalase detergent, Mammal, Skeleton specimen preparation

关键词: 兽类, 骨骼标本制作, 碱性蛋白酶消化