ELECTROPHO RETIC ANALYSIS OF HEMOGLOBIN ON THE HYBRID GENERATIONS OF WILD MOUSE AND LABORATORY MOUSE
ZHOU Yucan, HU Xiaomei
1984, 4(1):
53-61.
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To study penetic characteristics of the ariots proteins in the mise (Mus musculus),the hybridization of wild morse (grey fur) and latoratory mouEe(albino)was tested.lie;esvlts of hybridization and linkage of hemog-lobin with fur coolr locus are reported in this paper.The mating group,incltding 4 pairs of wild rouse(female)with laboratory mouse (male), has not genertated for 8 months.Cn the contrary,the other tra-ting group(4 pairs)of wild mouse (male)with latoratory mouse(ferrale)gene-rated offspring after about two months. The sum of F1 is 70 for 7 foetus in the latter group, and all individuals were of grey fur. It is evident that the grey fur is a dominant character and the white fur is a recessive character.Among 14 pairs of sib mating (F1), there are 7 pairs to generated offspring,the other 7 pairs are sterile,For fur,colour of F2 of 55 individuals the great malority are of grey fur,some are of white fur,only a few are of yellow or black fur. We regard all colour individuals as none albinos, then compared the sum of colour individuals with the those white ones(albinos). The ratio is 3.23:1,being agreed with Mendel's laws. There are also grey, white,yellow and black fur in the offspring of backcross. When the sum of colour individuals compare with the white ones,the ratio is 0.87:1,being in accordance with Mendel's laws,too.The hemoglobins of P,F1 and F2 were analysed by gel electrophoresis.It is found that in F1or F2, all hemoglobin patterns of grey fur are similar to those of wild mouse(male parant),in F2 all Hb patterns of white fur are not only similar to,but also identical with those of laboratory mouse(female parant).For example,when Hb of female parant is "single type",the Hb of F2(white fur) is also "single type"; when Hb of female parant is "diffuse type",the Hb of F2(white fur)is"diffuse type",too.It is obvious that linkage exists in the hereditary chaacters between herropobin and fur colour.Among Hb of hybrid,the new component of Hb vas not yet found. However,due to diffeience of beterozyosity of F1,the two molymorghic types of Hb are appeared.It is just a reflection of sex difference in F1. Similarly,due to difference of segregation of F2 the Hb polymorphism also occurred.For grey,yellow and black fur mice,the difference of Hb is not yet observed. Comparisicn on all patterns of Hb ccnfirrred that slow migrating components are probably important to decide what the fur colovr cf mouse will be.