关键词: 黑叶猴, 线粒体控制区, 单倍型, 遗传多样性

Abstract:

 Northeastern Guizhou is one of the main distribution areas of the François’ langur (Trachypithecus francoisi) in China, however, the habitat has been seriously damaged and population size of the François’ langur has declined very quickly, showing a distribution peculiarity of fragmentation in the Northeastern Guizhou. Therefore, understanding the morphological and molecular variation of different geographic groups of the François’ langur in these areas is of vital importance in designing conservation strategies. We used 79 faecal samples to investigate their mitochondrial DNA D-loop, all of which were collected from the wild François’ langur population in Northeastern Guizhou An additional 22 feces samples and 5 hair samples which were collected from the captive population in the Qianling Park of Guiyang were also tested. Finally, we obtained 88 sequences of this region (D-loop), containing 395 base pair fragment nucleotides. We identified 30 variable nucleotide sites of transitions and transversions, whereas no insertions/deletions was detected. 6 haplotypes were defined and used to construct the population phylogenetic tree, which revealed the François’ langur as an independent unit. This is helpful for studying the genetic diversity and conservation status of the wild Trachypithecus francoisi in Northeast Guizhou, and important for designing conservation strategies based on the evaluation of their adaptation ability and evolutionary potential.

Key words: Trachypithecus francoisi, Mitochondrial Control Region, Haplotye, genetic diversity