关键词: 大仓鼠, DNA 指纹谱, 探针, 微卫星, 反向杂交

Abstract: We extracted the genomic DNA from liver tissues of Tscherskia triton using isolation of high quality DNA method. Then the DNA template of Tscherskia triton was amplified with a single primer composing ( GTG)₅or (CA)₈ by using the polymerase
chain reaction (PCR). After agarose gel electrophoresis, fifteen specific amplification fragments were retrieved. Among these specific PCR products, we found that fragment (GTG)_{5 - 8,}(CA)_{8 - 1b} and (CA)_{8 - 5b} showed more positive signals than others in reverse hybridization test with genomic DNA. We designed the DIG labeled probes according to the sequencing result of these three fragments. The DNA fingerprints pattern of the greater long-tailed hamster individuals from different geographic areas hybridized by these probes were individual-specific and population-polymorphic. Moreover in contrast to DNA fingerprints hybridized by conventional probes derived from other sources, such as 33.6 and 33.15, these probes detected medium level of variation in DNA fingerprints, which were easier to score.

Key words: DNA fingerprint, Greater Long2tailed hamster ( Tscherskia triton), Microsatellite, Probe, Reverse hybridization