关键词: 豚鹿, 微卫星, 亲子鉴定, 谱系

Abstract: The hog deer (Axis porcinus) is a Class 1 National Key Protected Species in China;the species has been extirpated within China. China has a captive population of only about 30,of which the Chengdu Zoo has 22. It is critical for captive management within China to identify paternity relationships and establish the pedigree of the hog deer population in the Chengdu Zoo. We used 7 microsatellites to genotype 22 live and 5 dead hog deer individuals from the Chengdu Zoo;we identified potential 13 paternal relationships for animals in which maternity was already known. The exclusion method defined 8 of the 13 father - offspring pairs. The likelihood method determined the remaining 5 pairs with a confidence level of 95% . Combining the result of this paternity test with the breeding records of the Chengdu Zoo,we constructed the genetic pedigree for this hog deer population. Our result will serve as an important reference for the future artificial breeding strategy and management of the captive hog deer population in China.

Key words: Hog deer (Axis porcinus), Microsatellite, Paternity identification, Pedigree