关键词: 高原鼠兔, 乳酸脱氢酶, 同工酶, 高原适应, 生物化学

Abstract: The lactate dehydrogenase(LDH) isoenzymes from heart, liver, kidney and skeletal muscle in the pika (Ochotona curzcniae) were analysed by means of the polyacrylamide gel electrophoresis and the ultraviolet enzymatic determination at a simulated altitude of 5,000m for 15 days. The following conclusions were obtained:1. Pikas were exposed to a simulated altitude of 5,000 m for 15 days. Their changes of M-subunit ratio in crude extract of the heart, liver and kidney were no significance in the electrophoretic isoenzyme patterns of LDH, as compare with those of alt. 2300m.2. The exposure of the pika to simulated altitude oxygen conditions for 15 days resulted in a significant alteration in skeletal muscle LDH isoenzyme patterns. The proportion of M-subunit of LDH increased approximately by 14% from 2,300m to 5,000m altitude.3. Continuous exposure of the pika to a simulated altitude of 5,000 m for 15 days seems to be no effect on the LDH activities of heart, liver, kidney and skeletal muscle (P>0.5).

Key words: Pika, Ochotona curzoniae, Lactate dehydrogenase, LDH, Isoenzyme, Altitude acclimatization, Biochemistry