食物中蛋氨酸含量对高原鼠兔肠道微生物群落的影响

doi:10.16829/j.slxb.150867

兽类学报 ›› 2024, Vol. 44 ›› Issue (4): 411-426.DOI: 10.16829/j.slxb.150867

食物中蛋氨酸含量对高原鼠兔肠道微生物群落的影响

董昕^1,2, 陈慧青^1,2, 张璐^1,2, 尚国珍^1,3, 吴雁⁴, 边疆晖^1,3

1 中国科学院西北高原生物研究所, 高原生物适应与进化重点实验室, 西宁 810001;
2 中国科学院大学, 北京 100049;
3 青海省动物生态基因组学重点实验室, 西宁 810001;
4 杭州师范大学生命科学学院, 杭州 310012

收稿日期:2023-10-12 修回日期:2024-01-29 出版日期:2024-07-30 发布日期:2024-08-03
通讯作者: 尚国珍，E-mail:shangguozhen@nwipb.cas.cn
作者简介:董昕(2000-)，女，硕士研究生，主要从事动物生理生态学研究；陈慧青(1996-)，女，博士研究生，主要从事小哺乳动物种群生态学研究.
基金资助:
青海省自然科学基金青年项目(2021-ZJ-945Q)；青海省人民政府中国科学院三江源国家公园联合专项(LHZX-2021-05)

Effects of methionine contents in food on the gut microbiota of plateau pika

DONG Xin^1,2, CHEN Huiqing^1,2, ZHANG Lu^1,2, SHANG Guozhen^1,3, WU Yan⁴, BIAN Jianghui^1,3

1 Key Laboratory of Adaptation and Evolution of Plateau, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China;
2 University of Chinese Academy of Science, Beijing 100049, China;
3 Qinghai Key Laboratory of Animal Ecological Genomics, Xining 810001, China;
4 School of Life and Environment Sciences, Hangzhou Normal University, Hangzhou 310012, China

Received:2023-10-12 Revised:2024-01-29 Online:2024-07-30 Published:2024-08-03

摘要/Abstract

摘要： 过度放牧引起的退化草地为高原鼠兔(Ochotona curzoniae)提供了丰富的蛋白质食物资源，而食物中必需氨基酸(尤其是蛋氨酸)含量的变化会影响宿主体内肠道微生物群落。本研究依据不同退化草地高原鼠兔胃内容物中的蛋氨酸含量，使用0.26%和0.45%蛋氨酸含量的兔颗粒饲料分组饲喂高原鼠兔，50 d后收集粪便，利用宏基因组学以及非靶向代谢组学分析，评估高蛋氨酸处理对高原鼠兔肠道微生物群落及其代谢产物的影响。结果表明，与低蛋氨酸组相比，高蛋氨酸组高原鼠兔肠道微生物群落的α、β多样性均无显著差异，但Candidatus_Saccharibacteria、软壁菌门(Tenericutes)等菌群的丰度显著增加，丁酸代谢、牛磺酸和次牛磺酸代谢等菌群功能显著表达，其上调的代谢产物主要在初级胆汁酸合成、抗坏血酸和醛酸代谢、Th17细胞分化、牛磺酸和次牛磺酸代谢、B细胞受体信号通路、T细胞受体信号通路、NF-κB途径等通路富集；而低蛋氨酸组高原鼠兔菌群的糖类代谢功能显著表达，其上调的代谢产物主要在氨基酸代谢相关通路富集。上述结果表明，高蛋氨酸食物可改变高原鼠兔肠道微生物及其代谢产物，进而可能增强宿主的免疫和繁殖性能，对提高高原鼠兔个体适合度及其种群数量有重要的作用。

关键词: 高原鼠兔, 蛋氨酸, 肠道微生物, 宏基因组学, 非靶向代谢组学

Abstract: The degradation of grasslands caused by overgrazing provides abundant protein food resources for the plateau pika (Ochotona curzoniae) and changes in the content of essential amino acids (especially methionine) in the food can affect the host intestinal microbial community. In this study, plateau pikas were fed with 0. 26% and 0. 45% methionine diets according to the methionine content in their stomach contents in different degraded grasslands for 50 days. Collected feces were used for metagenomics and non-targeted metabolomics analysis to evaluate the effects of high methionine treatment on intestinal microbial communities and metabolites of plateau pika. The results showed that there was no significant difference in the α and β diversity of the intestinal microbial community of plateau pikas in the high methionine group compared with the low methionine group, but the abundance of Candidatus_Saccharibacteria and Tenericutes was significantly increased and the functions of butyric acid metabolism, taurine, and hypotaurine metabolism were significantly expressed, and the up-regulated metabolites were mainly enriched in primary bile acid synthesis, ascorbic acid and aldehyde metabolism, Th17 cell differentiation, taurine and hypotaurine metabolism, B cell receptor signaling pathway, T cell receptor signaling pathway, and NF-κB pathway. On the other hand, the carbohydrate metabolism function of plateau pika microbiota in the low methionine group was significantly expressed, and its up-regulated metabolites were mainly enriched in amino acid metabolism-related pathways. These results suggest that high methionine foods can change intestinal microbes and their metabolites, which may enhance the immune and reproductive performance of the host, and play an important role in improving the individual fitness and population size of plateau pikas.

Key words: Plateau pika (Ochotona curzoniae), Methionine, Gut microbes, Metagenomics, Non-targeted metabolomics

中图分类号:

Q494

董昕, 陈慧青, 张璐, 尚国珍, 吴雁, 边疆晖. 食物中蛋氨酸含量对高原鼠兔肠道微生物群落的影响[J]. 兽类学报, 2024, 44(4): 411-426.

DONG Xin, CHEN Huiqing, ZHANG Lu, SHANG Guozhen, WU Yan, BIAN Jianghui. Effects of methionine contents in food on the gut microbiota of plateau pika[J]. ACTA THERIOLOGICA SINICA, 2024, 44(4): 411-426.

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食物中蛋氨酸含量对高原鼠兔肠道微生物群落的影响

Effects of methionine contents in food on the gut microbiota of plateau pika

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