The complex burrowing system of Plateau Zokor has an important influence on soil physical and chemical characteristics in alpine meadows. Soil samples taken at burrow sites of zokors were compared with those of non-burrow soil samples collected in the same soil layer in surrounding areas without disturbance oby zokors. We measured soil microorganisms (bacteria, fungi, actinomycetes), soil enzymes (urease, invertase, alkaline phosphatase) and soil total nitrogen and organic matter, as well as available phosphorus in active burrow and non-burrow soil .Our results showed that the content of available phosphorus and total nitrogen in burrows of zokor were higher than those of non-burrow soil; the content of available phosphorus differed significantly in May and October (P<0.05); the content of organic matter and fungi in non-burrow soil were significantly higher than that of burrows (P<0.05); there were no significant differences in activities of three soil enzymes between active burrow and non-burrow soil (P>0.05); the content of soil bacteria in May and fungi in October in non-burrow soil were significantly higher than that of the burrows (P<0.05); soil bacteria, actinomycetes, available phosphorus, total nitrogen and organic matter reached maximum values in both the burrow and non-burrow soil in August , these indices were not significantly different between burrow and non-burrow soil in the other months (P>0.05). Based on the above results, we concluded that the soil microorganism and soil enzyme were not significantly different between burrow and non-burrow soil (P>0.05).